skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Initial characterization of receptors for molecules that induce the settlement and metamorphosis of Haliotis rufescens larvae

Abstract

Larvae of the marine gastropod mollusc Haliotis refescens are induced to undergo metamorphosis by ..gamma..-aminobutyric acid (GABA) and stereochemically related compounds. The most potent of these inducers is (-)-..beta..-(parachlorophenyl)-GABA (baclofen). The inductive response exhibits positive cooperatively, and is subject to both facilitation (up-regulation) and habituation (down-regulation). Facilitation is brought about by diamino acids such as L-diaminopropionic acid (L-DAPA), and is characterized by decreased Hill coefficients (n/sub H/) and concentration requirements (EC/sub 50/) for inducers. Facilitation does not require the simultaneous presence of facilitating and inducing compounds, and the facilitated state is persistent. Larvae are capable of being up-regulated 2 days before they are capable of undergoing settlement and metamorphosis. Habituation can be brought about by exposure of pre-competent larvae to GABA 4 days prior to the attainment of competence; it is then slowly reversible. Larvae specifically bind tritiated (-)-baclofen in a manner that is saturable with both increasing time of exposure of larvae to, and with increasing concentration of, this compound. Specific binding can be competed for by unlabeled GABA-mimetic inducing molecules; the order of effectiveness of these molecules as competitors for specific binding correlates well with their effectiveness as inducers of metamorphosis. Facilitation of larvae by exposure to diaminomore » acids does not alter their specific binding of tritiated (-)-baclofen. It is concluded from these findings that Haliotis larvae possess receptors for GABA-mimetic compounds.« less

Authors:
Publication Date:
Research Org.:
California Univ., Santa Barbara (USA)
OSTI Identifier:
6700840
Alternate Identifier(s):
OSTI ID: 6700840
Resource Type:
Thesis/Dissertation
Resource Relation:
Other Information: Thesis (Ph. D.)
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; AMINOBUTYRIC ACID; RADIORECEPTOR ASSAY; METAMORPHOSIS; CHEMICAL ACTIVATION; LARVAE; MOLLUSCS; RECEPTORS; TRITIUM COMPOUNDS; AMINO ACIDS; ANIMALS; AQUATIC ORGANISMS; AUTONOMIC NERVOUS SYSTEM AGENTS; CARBOXYLIC ACIDS; DRUGS; INVERTEBRATES; ISOTOPE APPLICATIONS; LABELLED COMPOUNDS; MEMBRANE PROTEINS; NEUROREGULATORS; ORGANIC ACIDS; ORGANIC COMPOUNDS; PROTEINS; TRACER TECHNIQUES 550601* -- Medicine-- Unsealed Radionuclides in Diagnostics

Citation Formats

Trapido-Rosenthal, H.G. Initial characterization of receptors for molecules that induce the settlement and metamorphosis of Haliotis rufescens larvae. United States: N. p., 1985. Web.
Trapido-Rosenthal, H.G. Initial characterization of receptors for molecules that induce the settlement and metamorphosis of Haliotis rufescens larvae. United States.
Trapido-Rosenthal, H.G. Tue . "Initial characterization of receptors for molecules that induce the settlement and metamorphosis of Haliotis rufescens larvae". United States.
@article{osti_6700840,
title = {Initial characterization of receptors for molecules that induce the settlement and metamorphosis of Haliotis rufescens larvae},
author = {Trapido-Rosenthal, H.G.},
abstractNote = {Larvae of the marine gastropod mollusc Haliotis refescens are induced to undergo metamorphosis by ..gamma..-aminobutyric acid (GABA) and stereochemically related compounds. The most potent of these inducers is (-)-..beta..-(parachlorophenyl)-GABA (baclofen). The inductive response exhibits positive cooperatively, and is subject to both facilitation (up-regulation) and habituation (down-regulation). Facilitation is brought about by diamino acids such as L-diaminopropionic acid (L-DAPA), and is characterized by decreased Hill coefficients (n/sub H/) and concentration requirements (EC/sub 50/) for inducers. Facilitation does not require the simultaneous presence of facilitating and inducing compounds, and the facilitated state is persistent. Larvae are capable of being up-regulated 2 days before they are capable of undergoing settlement and metamorphosis. Habituation can be brought about by exposure of pre-competent larvae to GABA 4 days prior to the attainment of competence; it is then slowly reversible. Larvae specifically bind tritiated (-)-baclofen in a manner that is saturable with both increasing time of exposure of larvae to, and with increasing concentration of, this compound. Specific binding can be competed for by unlabeled GABA-mimetic inducing molecules; the order of effectiveness of these molecules as competitors for specific binding correlates well with their effectiveness as inducers of metamorphosis. Facilitation of larvae by exposure to diamino acids does not alter their specific binding of tritiated (-)-baclofen. It is concluded from these findings that Haliotis larvae possess receptors for GABA-mimetic compounds.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jan 01 00:00:00 EST 1985},
month = {Tue Jan 01 00:00:00 EST 1985}
}

Thesis/Dissertation:
Other availability
Please see Document Availability for additional information on obtaining the full-text document. Library patrons may search WorldCat to identify libraries that hold this thesis or dissertation.

Save / Share: