DNA nucleoside composition and methylation in several species of microalgae
- National Renewable Energy Lab., Golden, CO (United States)
Total DNA was isolated from 10 species of microalgae, including representatives of the Chlorophyceae (Chlorella ellipsoidea, Chlamydomonas reinhardtii, and Monoraphidium minutum), Bacillariophyceae (Cyclotella cryptica, Navicula saprophila, Nitzschia pusilla, and Phaeodactylum tricornutum), Charophyceae (Stichococcus sp.), Dinophyceae (Crypthecodinium cohnii), and Prasinophyceae (Tetraselmis suecica). Control samples of Escherichia coli and calf thymus DNA were also analyzed. The nucleoside base composition of each DNA sample was determined by reversed-phase high performance liquid chromatography. All samples contained 5-methyldeoxycytidine, although at widely varying levels. In M. minutum, about one-third of the cytidine residues were methylated. Restriction analysis supported this high degree of methylation in M. minutum and suggested that methylation is biased toward 5[prime]-CG dinucleotides. The guanosine + cytosine (GC) contents of the green algae were, with the exception of Stichococcus sp., consistently higher than those of the diatoms. Monoraphidium minutum exhibited an extremely high GC content of 71%. Such a value is rare among eukaryotic organisms and might indicate an unusual codon usage. This work is important for developing strategies for transformation and gene cloning in these algae. 46 refs., 1 fig., 2 tabs.
- OSTI ID:
- 6698110
- Journal Information:
- Journal of Phycology; (United States), Vol. 28:3; ISSN 0022-3646
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ALGAE
CLONING
DNA
METHYLATION
CHLAMYDOMONAS
CHLORELLA
CYTIDINE
CYTOSINE
DIATOMS
ESCHERICHIA COLI
GENES
GUANOSINE
NUCLEOSIDES
AMINES
AROMATICS
AZAARENES
AZINES
BACTERIA
CHEMICAL REACTIONS
CHLOROPHYCOTA
CHROMOPHYCOTA
HETEROCYCLIC COMPOUNDS
MICROORGANISMS
NUCLEIC ACIDS
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PLANTS
PURINES
PYRIMIDINES
RIBOSIDES
UNICELLULAR ALGAE
550200* - Biochemistry