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Title: Molecular and cytogenetic characterization of a chinese hamster/human hybrid cell line containing a der (21)t(Ypter [yields] cenY::cen21 [yields] 21qter) chromosome

Journal Article · · Genomics; (United States)
; ;  [1];  [2]; ;  [3];  [4];  [5]
  1. Eleanor Roosevelt Institute, Denver, CO (United States)
  2. Univ. of Arizona, Tucson (United States)
  3. Institute of Human Genetics, Rome (Italy)
  4. Dept. of Genetics and Microbiology, Pavia (Italy)
  5. Eleanor Roosevelt Institute, Denver, CO (United States) Institute of Human Genetics, Rome (Italy)
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Human/rodent somatic cell hybrids have been exceedingly useful in assigning human genes and DNA sequences to specific human chromosomes. As new technologies for analyzing the human chromosome complement of such human/rodent hybrid cells become available, it is of critical importance that these be applied to enhance characterization of existing hybrids. This is particularly important since human chromosomes in such hybrids have been observed to rearrange with time. We report here the use of fluorescence in situ hybridization of DNA probes to metaphase chromosomes to analyze one hybrid designated 72532X6. This analysis shows that the chromosome suspected to be a normal human chromosome 21 in this hybrid is actually a translocation chromosome containing Yp and 21 q. In addition, the hybrid contains a fragment of human chromosome 9 translocated to a Chinese hamster chromosome. Analysis of the chromosomes from the human donor indicates that his chromosomes are normal. Thus, this translocation chromosome appears to have arisen after formation of the hybrid. 14 refs., 2 figs.

OSTI ID:
6697338
Journal Information:
Genomics; (United States), Vol. 15:1; ISSN 0888-7543
Country of Publication:
United States
Language:
English

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
HUMAN CHROMOSOMES
GENE RECOMBINATION
TRANSLOCATION
CHROMOSOMES
DNA
DNA HYBRIDIZATION
FLUORESCENCE
GENES
GENETIC MAPPING
HUMAN CHROMOSOME 21
HUMAN Y CHROMOSOME
HYBRIDIZATION
PROBES
HETEROCHROMOSOMES
LUMINESCENCE
MAPPING
NUCLEIC ACIDS
ORGANIC COMPOUNDS
Y CHROMOSOME
550400* - Genetics