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Title: Effects of forskolin on cerebral blood flow: implications for a role of adenylate cyclase

Abstract

We have studied cerebral vascular effects of forskolin, a drug which stimulates adenylate cyclase and potentiates dilator effects of adenosine in other vascular beds. Our goals were to determine whether forskolin is a cerebral vasodilator and whether it potentiates cerebral vasodilator responses to adenosine. We measured cerebral blood flow with microspheres in anesthetized rabbits. Forskolin (10 micrograms/kg per min) increased blood flow (ml/min per 100 gm) from 39 +/- 5 (mean +/- S.E.) to 56 +/- 9 (p less than 0.05) in cerebrum, and increased flow to myocardium and kidney despite a decrease in mean arterial pressure. Forskolin did not alter cerebral oxygen consumption, which indicates that the increase in cerebral blood flow is a direct vasodilator effect and is not secondary to increased metabolism. We also examined effects of forskolin on the response to infusion of adenosine. Cerebral blood flow was measured during infusion of 1-5 microM/min adenosine into one internal carotid artery, under control conditions and during infusion of forskolin at 3 micrograms/kg per min i.v. Adenosine alone increased ipsilateral cerebral blood flow from 32 +/- 3 to 45 +/- 5 (p less than 0.05). Responses to adenosine were not augmented during infusion of forskolin. We conclude thatmore » forskolin is a direct cerebral vasodilator and forskolin does not potentiate cerebral vasodilator responses to adenosine.« less

Authors:
; ;
Publication Date:
Research Org.:
VA Medical Center, Iowa City, IA
OSTI Identifier:
6695973
Alternate Identifier(s):
OSTI ID: 6695973
Resource Type:
Journal Article
Journal Name:
Stroke; (United States)
Additional Journal Information:
Journal Volume: 6
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CEREBRUM; BLOOD FLOW; KIDNEYS; MYOCARDIUM; VASODILATORS; BIOLOGICAL EFFECTS; ACETYLCHOLINE; ADENOSINE; DRUGS; MICROSPHERES; RABBITS; VASODILATION; AMINES; AMMONIUM COMPOUNDS; ANIMALS; AUTONOMIC NERVOUS SYSTEM AGENTS; BODY; BRAIN; CARDIOVASCULAR AGENTS; CARDIOVASCULAR SYSTEM; CENTRAL NERVOUS SYSTEM; ESTERS; HEART; MAMMALS; MUSCLES; NERVOUS SYSTEM; NEUROREGULATORS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC COMPOUNDS; ORGANS; PARASYMPATHOMIMETICS; QUATERNARY COMPOUNDS; RIBOSIDES; VERTEBRATES 551001* -- Physiological Systems-- Tracer Techniques

Citation Formats

Wysham, D.G., Brotherton, A.F., and Heistad, D.D. Effects of forskolin on cerebral blood flow: implications for a role of adenylate cyclase. United States: N. p., 1986. Web. doi:10.1161/01.STR.17.6.1299.
Wysham, D.G., Brotherton, A.F., & Heistad, D.D. Effects of forskolin on cerebral blood flow: implications for a role of adenylate cyclase. United States. doi:10.1161/01.STR.17.6.1299.
Wysham, D.G., Brotherton, A.F., and Heistad, D.D. Sat . "Effects of forskolin on cerebral blood flow: implications for a role of adenylate cyclase". United States. doi:10.1161/01.STR.17.6.1299.
@article{osti_6695973,
title = {Effects of forskolin on cerebral blood flow: implications for a role of adenylate cyclase},
author = {Wysham, D.G. and Brotherton, A.F. and Heistad, D.D.},
abstractNote = {We have studied cerebral vascular effects of forskolin, a drug which stimulates adenylate cyclase and potentiates dilator effects of adenosine in other vascular beds. Our goals were to determine whether forskolin is a cerebral vasodilator and whether it potentiates cerebral vasodilator responses to adenosine. We measured cerebral blood flow with microspheres in anesthetized rabbits. Forskolin (10 micrograms/kg per min) increased blood flow (ml/min per 100 gm) from 39 +/- 5 (mean +/- S.E.) to 56 +/- 9 (p less than 0.05) in cerebrum, and increased flow to myocardium and kidney despite a decrease in mean arterial pressure. Forskolin did not alter cerebral oxygen consumption, which indicates that the increase in cerebral blood flow is a direct vasodilator effect and is not secondary to increased metabolism. We also examined effects of forskolin on the response to infusion of adenosine. Cerebral blood flow was measured during infusion of 1-5 microM/min adenosine into one internal carotid artery, under control conditions and during infusion of forskolin at 3 micrograms/kg per min i.v. Adenosine alone increased ipsilateral cerebral blood flow from 32 +/- 3 to 45 +/- 5 (p less than 0.05). Responses to adenosine were not augmented during infusion of forskolin. We conclude that forskolin is a direct cerebral vasodilator and forskolin does not potentiate cerebral vasodilator responses to adenosine.},
doi = {10.1161/01.STR.17.6.1299},
journal = {Stroke; (United States)},
number = ,
volume = 6,
place = {United States},
year = {1986},
month = {11}
}