The inhibition of mouse L-cell 45 S ribosomal RNA processing is a highly uv-resistant property of vesicular stomatitis virus

Journal Article · Sun Jul 01 00:00:00 EDT 1990 · Virology; (USA)

DOI:https://doi.org/10.1016/0042-6822(90)90461-Y· OSTI ID:6695360

Zan, M; Evans, P; Lucas-Lenard, J ^[1]

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1. Univ. of Connecticut, Storrs (USA)

In mouse L cells infected with vesicular stomatitis virus (VSV), the synthesis of 45 S rRNA and its conversion to 28 S and 18 S rRNA are inhibited during the course of infection. Evidence is presented that the lack of accumulation of stable rRNA species results not only from the decreased transcription and processing of 45 S rRNA, but also from an increased breakdown of pre-rRNA or stable rRNA during processing. In cells prelabeled with (3H)uridine and then infected, the 28 S and 18 S rRNA species remain unaffected. Studies using uv-irradiated VSV indicate that the viral function involved in rRNA synthesis inhibition is slightly more sensitive to uv irradiation than the function involved in processing inhibition. These results suggest that the VSV functions involved in 45 S rRNA synthesis and processing inhibition may be related, or overlapping, but not identical. In cells infected by VSV mutant T1026R1, total RNA synthesis is inhibited, but the distribution of precursor and stable rRNA species remains nearly normal for up to 5 hr after infection. The function of the mutant virus involved in the inhibition of rRNA processing appears to be defective. In mengovirus-infected L cells, 45 S rRNA synthesis, but not processing, is severely inhibited soon after infection, indicating that a decrease in rRNA transcription is not necessarily accompanied by a decrease in processing.

• OSTI ID: 6695360
• Journal Information: Virology; (USA), Vol. 177:1; ISSN 0042-6822
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
RIBOSOMAL RNA
BIOSYNTHESIS
VIRAL DISEASES
PATHOGENESIS
VIRUSES
INFECTIVITY
ANIMAL CELLS
BIOLOGICAL RADIATION EFFECTS
CELL TRANSFORMATIONS
INHIBITION
MICE
PRECURSOR
RNA
TRACER TECHNIQUES
TRANSCRIPTION
ULTRAVIOLET RADIATION
URIDINE
ANIMALS
AZINES
BIOLOGICAL EFFECTS
DISEASES
ELECTROMAGNETIC RADIATION
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INFECTIOUS DISEASES
ISOTOPE APPLICATIONS
MAMMALS
MICROORGANISMS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PARASITES
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
RIBOSIDES
RODENTS
SYNTHESIS
URACILS
VERTEBRATES
550901* - Pathology- Tracer Techniques
560130 - Radiation Effects on Microorganisms