Eukaryotic transcription and processing: regulation of gene expression. Progress report, November 1, 1977--October 31, 1978
We are attempting to prepare a cell-free protein synthesizing system from corn embryos that will translate mRNA's isolated from corn grain or transcribed from corn DNA by corn RNA polymerase II. The experience of others utilizing heterologous translation systems and isolated plant mRNA fractions suggests that the extent as well as the nature of the RNA sequences translated into peptide sequences may be influenced by components in the heterologous system. We wish to prepare a homologous system to compare with a heterologous system for detection and identification of potentially regulatory components required for in vitro polypeptide synthesis. The notion is that subtle differences in mRNA recognition by homologous versus heterologous components in the in vitro translation systems may be identified by comparison of the products accumulated by each system translating the same mRNA. Potentially the approach provides both an assay and a source of the putative ''regulatory components.'' To this end we have undertaken: establishment of an active wheat germ translating system, reestablishment of the maize seedling amino acid incorporating system and its conversion to an S23 translator of endogeneous mRNAs, propagation of Barley Stripe Mosaic Virus in barley, isolation of its genome and resolution of the RNAs on a preparative scale, isolation of the poly A bearing RNA fraction from seedling and developing kernel systems, and isolation of the zein mRNAs from maize protein bodies.
- Research Organization:
- Florida Univ., Gainesville (USA). Dept. of Biochemistry and Molecular Biology
- DOE Contract Number:
- EY-76-S-05-3982
- OSTI ID:
- 6687013
- Report Number(s):
- ORO-3982-48
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BARLEY
GENETICS
DNA
BIOCHEMISTRY
MAIZE
MESSENGER-RNA
POLYPEPTIDES
BIOSYNTHESIS
PROTEINS
WHEAT
AMINO ACIDS
EMBRYOS
PEPTIDES
SEEDLINGS
BIOLOGY
BIOMASS
CARBOXYLIC ACIDS
CEREALS
CHEMISTRY
ENERGY SOURCES
GRAMINEAE
GRASS
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PLANTS
RENEWABLE ENERGY SOURCES
RNA
SYNTHESIS
550400* - Genetics
550200 - Biochemistry