Effect of transforming growth factor-alpha on inositol phospholipid metabolism in human epidermoid carcinoma cells
Transforming growth factor-alpha (TGF-alpha) stimulates (in a dose-dependent manner) the incorporation of (/sup 32/P)Pi into phosphatidylinositol (PI), phosphatidylinositol 4-phosphate (PIP), phosphatidylinositol 4,5-bisphosphate (PIP2), and phosphatidic acid (PA) in the human epidermoid carcinoma cell line (A431). The effect of TGF-alpha on the incorporation was found to be similar to that of EGF. On the other hand, a striking difference in the activation of diacylglycerol (DG) kinase activity was seen between TGF-alpha and EGF. At least 100 times more TGF-alpha was required to achieve maximal stimulation of DG kinase activity relative to EGF. These results suggest that the activation of DG kinase by TGF-alpha may involve a mechanism independent from or subsequent to activation of the EGF receptor.
- Research Organization:
- Tokyo Metropolitan Institute of Gerontology (Japan)
- OSTI ID:
- 6681606
- Journal Information:
- J. Cell. Biochem.; (United States), Vol. 37:4
- Country of Publication:
- United States
- Language:
- English
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PHOSPHOLIPIDS
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PHOSPHOTRANSFERASES
ENZYME ACTIVITY
CARCINOMAS
DOSE-RESPONSE RELATIONSHIPS
MAN
PHOSPHATES
PHOSPHORUS 32
TRACER TECHNIQUES
TUMOR CELLS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
DISEASES
ENZYMES
ESTERS
ISOTOPE APPLICATIONS
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KINETICS
LIGHT NUCLEI
LIPIDS
MAMMALS
MITOGENS
NEOPLASMS
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
OXYGEN COMPOUNDS
PHOSPHORUS COMPOUNDS
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
PRIMATES
PROTEINS
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REACTION KINETICS
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550501* - Metabolism- Tracer Techniques