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Title: In situ hybridization of nucleus basalis neurons shows increased. beta. -amyloid mRNA in Alzheimer disease

Abstract

To determine which cells within the brain produce ..beta..-amyloid mRNA and to assess expression of the ..beta..-amyloid gene in Alzheimer disease, the authors analyzed brain tissue from Alzheimer and control patients by in situ hybridization. The results demonstrate that ..beta..-amyloid mRNA is produced by neurons in the nucleus basalis of Meynert and cerebral cortex and that nuclues basalis perikarya from Alzheimer patients consistently hybridize more ..beta..-amyloid probe than those from controls. These observations support the hypothesis that increased expression of the ..beta..-amyloid gene plays an important role in the deposition of amyloid in the brains of patients with Alzheimer disease.

Authors:
; ; ; ;
Publication Date:
Research Org.:
Case Western Reserve Univ., Cleveland, OH (USA)
OSTI Identifier:
6659345
Alternate Identifier(s):
OSTI ID: 6659345
Resource Type:
Journal Article
Resource Relation:
Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States); Journal Volume: 85:4
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; NERVE CELLS; AUTORADIOGRAPHY; NERVOUS SYSTEM DISEASES; PATHOGENESIS; OLIGONUCLEOTIDES; HYBRIDIZATION; BRAIN; GENE REGULATION; GENES; IODINE 125; KIDNEYS; LIVER; MESSENGER-RNA; PROTEINS; ANIMAL CELLS; BETA DECAY RADIOISOTOPES; BODY; CENTRAL NERVOUS SYSTEM; DAYS LIVING RADIOISOTOPES; DIGESTIVE SYSTEM; DISEASES; ELECTRON CAPTURE RADIOISOTOPES; GLANDS; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPES; NERVOUS SYSTEM; NUCLEI; NUCLEIC ACIDS; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANS; RADIOISOTOPES; RNA; SOMATIC CELLS 550201* -- Biochemistry-- Tracer Techniques

Citation Formats

Cohen, M.L., Golde, T.E., Usiak, M.F., Younkin, L.H., and Younkin, S.G. In situ hybridization of nucleus basalis neurons shows increased. beta. -amyloid mRNA in Alzheimer disease. United States: N. p., 1988. Web. doi:10.1073/pnas.85.4.1227.
Cohen, M.L., Golde, T.E., Usiak, M.F., Younkin, L.H., & Younkin, S.G. In situ hybridization of nucleus basalis neurons shows increased. beta. -amyloid mRNA in Alzheimer disease. United States. doi:10.1073/pnas.85.4.1227.
Cohen, M.L., Golde, T.E., Usiak, M.F., Younkin, L.H., and Younkin, S.G. Mon . "In situ hybridization of nucleus basalis neurons shows increased. beta. -amyloid mRNA in Alzheimer disease". United States. doi:10.1073/pnas.85.4.1227.
@article{osti_6659345,
title = {In situ hybridization of nucleus basalis neurons shows increased. beta. -amyloid mRNA in Alzheimer disease},
author = {Cohen, M.L. and Golde, T.E. and Usiak, M.F. and Younkin, L.H. and Younkin, S.G.},
abstractNote = {To determine which cells within the brain produce ..beta..-amyloid mRNA and to assess expression of the ..beta..-amyloid gene in Alzheimer disease, the authors analyzed brain tissue from Alzheimer and control patients by in situ hybridization. The results demonstrate that ..beta..-amyloid mRNA is produced by neurons in the nucleus basalis of Meynert and cerebral cortex and that nuclues basalis perikarya from Alzheimer patients consistently hybridize more ..beta..-amyloid probe than those from controls. These observations support the hypothesis that increased expression of the ..beta..-amyloid gene plays an important role in the deposition of amyloid in the brains of patients with Alzheimer disease.},
doi = {10.1073/pnas.85.4.1227},
journal = {Proc. Natl. Acad. Sci. U.S.A.; (United States)},
number = ,
volume = 85:4,
place = {United States},
year = {Mon Feb 01 00:00:00 EST 1988},
month = {Mon Feb 01 00:00:00 EST 1988}
}