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Title: Lung-derived growth factors: possible paracrine effectors of fetal lung development

Abstract

A potential role for paracrine secretions in lung organogenesis has been hypothesized (Alescio and Piperno, 1957). These studies present direct support for the paracrine model by demonstrating the presence of locally produced mitogenic/maturational factors in fetal rat lung tissue. Conditioned serum free medium (CSFM) from nineteen-day fetal rat lung cultures was shown to contain several bioactive peptides as detected by /sup 3/H-Thymidine incorporation into chick embryo and rat lung fibroblasts, as well as /sup 14/C-choline incorporation into surfactant in mixed cell cultures. Using ion-exchange chromatography and Sephadex gel filtration, a partially purified mitogen, 11-III, was obtained. The partially purified 11-III stimulates mitosis in chick embryo fibroblasts and post-natal rat lung fibroblasts. Multiplication in fetal rat lung fibroblasts cultures is stimulated only when these are pre-incubated with a competence factor or unprocessed CSFM. This suggests the existence of an endogenously produced competence factor important in the regulation of fetal lung growth. Preparation 11-III does not possess surfactant stimulating activity as assessed by /sup 3/H-choline incorporation into lipids in predominantly type-II cell cultures. These data demonstrate the presence of a maturational/mitogenic factor, influencing type-II mixed cell cultures. In addition, 11-III had been shown to play an autocrine role stimulating the proliferation ofmore » fetal lung fibroblasts. Finally, these data suggest the existence of a local produced competence factor.« less

Authors:
Publication Date:
Research Org.:
Hawaii Univ., Honolulu (USA)
OSTI Identifier:
6655162
Resource Type:
Thesis/Dissertation
Resource Relation:
Other Information: Thesis (Ph. D.)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CHOLINE; UPTAKE; GROWTH; STIMULATION; LUNGS; MORPHOLOGICAL CHANGES; RATS; FETUSES; THYMIDINE; CARBON 14 COMPOUNDS; CELL CULTURES; FIBROBLASTS; GEL PERMEATION CHROMATOGRAPHY; ION EXCHANGE CHROMATOGRAPHY; TRACER TECHNIQUES; TRITIUM COMPOUNDS; ALCOHOLS; AMINES; AMMONIUM COMPOUNDS; ANIMAL CELLS; ANIMALS; AZINES; BODY; CHROMATOGRAPHY; CONNECTIVE TISSUE CELLS; DRUGS; HETEROCYCLIC COMPOUNDS; HYDROXY COMPOUNDS; ISOTOPE APPLICATIONS; LABELLED COMPOUNDS; LIPOTROPIC FACTORS; MAMMALS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANS; PYRIMIDINES; QUATERNARY COMPOUNDS; RESPIRATORY SYSTEM; RIBOSIDES; RODENTS; SEPARATION PROCESSES; SOMATIC CELLS; VERTEBRATES 550301* -- Cytology-- Tracer Techniques; 550801 -- Morphology-- Tracer Techniques

Citation Formats

Montes, A.M. Lung-derived growth factors: possible paracrine effectors of fetal lung development. United States: N. p., 1985. Web.
Montes, A.M. Lung-derived growth factors: possible paracrine effectors of fetal lung development. United States.
Montes, A.M. 1985. "Lung-derived growth factors: possible paracrine effectors of fetal lung development". United States. doi:.
@article{osti_6655162,
title = {Lung-derived growth factors: possible paracrine effectors of fetal lung development},
author = {Montes, A.M.},
abstractNote = {A potential role for paracrine secretions in lung organogenesis has been hypothesized (Alescio and Piperno, 1957). These studies present direct support for the paracrine model by demonstrating the presence of locally produced mitogenic/maturational factors in fetal rat lung tissue. Conditioned serum free medium (CSFM) from nineteen-day fetal rat lung cultures was shown to contain several bioactive peptides as detected by /sup 3/H-Thymidine incorporation into chick embryo and rat lung fibroblasts, as well as /sup 14/C-choline incorporation into surfactant in mixed cell cultures. Using ion-exchange chromatography and Sephadex gel filtration, a partially purified mitogen, 11-III, was obtained. The partially purified 11-III stimulates mitosis in chick embryo fibroblasts and post-natal rat lung fibroblasts. Multiplication in fetal rat lung fibroblasts cultures is stimulated only when these are pre-incubated with a competence factor or unprocessed CSFM. This suggests the existence of an endogenously produced competence factor important in the regulation of fetal lung growth. Preparation 11-III does not possess surfactant stimulating activity as assessed by /sup 3/H-choline incorporation into lipids in predominantly type-II cell cultures. These data demonstrate the presence of a maturational/mitogenic factor, influencing type-II mixed cell cultures. In addition, 11-III had been shown to play an autocrine role stimulating the proliferation of fetal lung fibroblasts. Finally, these data suggest the existence of a local produced competence factor.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = 1985,
month = 1
}

Thesis/Dissertation:
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  • The concentration of disaturated phosphatidylcholine (DPPC) in 16 day lung tissue was measured after 5 days in culture. When grown in the absence of serum and hormones, levels of DPPC, assayed by phosphorus content, increased over 17 day in vivo controls. Treated with thyroxine and dexamethasone, DPPC levels were comparable to 2 day postnatal controls. Levels of DPPC increased in cultures containing dexamethasone alone while thyroxine alone had significantly less effect. 16- and 19-day fetal lung tissues were labeled with {sup 35}S-sulfate and {sup 3}H-glucosamine. Each pool was analyzed by DEAE-sepharose chromatography and by digestion with nitrous acid and chondroitinase.more » GAG synthesis was inhibited using {beta}-xyloside. The {beta}-xyloside inhibition of GAG synthesis was examined morphologically by transmission and scanning electron microscopy and functionally by autoradiography, sequential extraction, chromatography, and digestion as above.« less
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  • A monoclonal antibody, designated PR7212 (IgG1), has been developed with specifically recognizes a cell-surface receptor for platelet-derived growth factor (PDGF). The antibody recognizes an extracellular epitope of the receptor, demonstrated by its ability to bind to intact cells. Using this antibody I have detected three forms of the receptor of 180, 164, and 130 kDa. All three forms were detected by Western blot analysis of human dermal fiberblasts. Immunoprecipitates of {sup 32}P-labeled membrane extracts of human dermal fibroblasts demonstrate that phosphorylation of all three forms of the receptor is stimulated by PDGF. In addition, several smaller molecules were detected, rangingmore » in size from 113 to 49 kDa, which are also phosphorylated in response to PDGF addition. These smaller molecules may be either PDGF receptor kinase substrates or partially degraded receptor. Only the 180 and the 164 kDa forms of the receptor are detectable from immunoprecipitates of soluble extracts of {sup 35}S-metabolically labeled cells. Pulse-chase experiments demonstrate that the 164 kDa form is a precursor of the 180 kDa molecule.« less