Detection of human muscle glycogen by natural abundance /sup 13/C NMR
Abstract
Natural abundance /sup 13/C nuclear magnetic resonance spectroscopy was used to detect signals from glycogen in the human gastrocnemius muscle. The reproducibility of the measurement was demonstrated, and the ability to detect dynamic changes was confirmed by measuring a decrease in muscle glycogen levels after exercise and its subsequent repletion. Single frequency gated /sup 1/H decoupling was used to obtain decoupled natural abundance /sup 13/C NMR spectra of the C-1 position of muscle glycogen.
- Authors:
- Publication Date:
- Research Org.:
- Yale Univ. School of Medicine, New Haven, CT (USA)
- OSTI Identifier:
- 6651370
- Resource Type:
- Journal Article
- Journal Name:
- Proc. Natl. Acad. Sci. U.S.A.; (United States)
- Additional Journal Information:
- Journal Volume: 85:5
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; GLYCOGEN; NMR SPECTRA; MUSCLES; NUCLEAR MAGNETIC RESONANCE; CARBON 13; GLUCOSE; MAN; PROTONS; ALDEHYDES; ANIMALS; BARYONS; CARBOHYDRATES; CARBON ISOTOPES; ELEMENTARY PARTICLES; EVEN-ODD NUCLEI; FERMIONS; HADRONS; HEXOSES; ISOTOPES; LIGHT NUCLEI; MAGNETIC RESONANCE; MAMMALS; MONOSACCHARIDES; NUCLEI; NUCLEONS; ORGANIC COMPOUNDS; POLYSACCHARIDES; PRIMATES; RESONANCE; SACCHARIDES; SPECTRA; STABLE ISOTOPES; VERTEBRATES; 550501* - Metabolism- Tracer Techniques
Citation Formats
Avison, M J, Rothman, D L, Nadel, E, and Shulman, R G. Detection of human muscle glycogen by natural abundance /sup 13/C NMR. United States: N. p., 1988.
Web. doi:10.1073/pnas.85.5.1634.
Avison, M J, Rothman, D L, Nadel, E, & Shulman, R G. Detection of human muscle glycogen by natural abundance /sup 13/C NMR. United States. https://doi.org/10.1073/pnas.85.5.1634
Avison, M J, Rothman, D L, Nadel, E, and Shulman, R G. 1988.
"Detection of human muscle glycogen by natural abundance /sup 13/C NMR". United States. https://doi.org/10.1073/pnas.85.5.1634.
@article{osti_6651370,
title = {Detection of human muscle glycogen by natural abundance /sup 13/C NMR},
author = {Avison, M J and Rothman, D L and Nadel, E and Shulman, R G},
abstractNote = {Natural abundance /sup 13/C nuclear magnetic resonance spectroscopy was used to detect signals from glycogen in the human gastrocnemius muscle. The reproducibility of the measurement was demonstrated, and the ability to detect dynamic changes was confirmed by measuring a decrease in muscle glycogen levels after exercise and its subsequent repletion. Single frequency gated /sup 1/H decoupling was used to obtain decoupled natural abundance /sup 13/C NMR spectra of the C-1 position of muscle glycogen.},
doi = {10.1073/pnas.85.5.1634},
url = {https://www.osti.gov/biblio/6651370},
journal = {Proc. Natl. Acad. Sci. U.S.A.; (United States)},
number = ,
volume = 85:5,
place = {United States},
year = {Tue Mar 01 00:00:00 EST 1988},
month = {Tue Mar 01 00:00:00 EST 1988}
}
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