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Title: Cloning vector

Abstract

A vector comprising a filamentous phage sequence containing a first copy of filamentous phage gene X and other sequences necessary for the phage to propagate is disclosed. The vector also contains a second copy of filamentous phage gene X downstream from a promoter capable of promoting transcription in a bacterial host. In a preferred form of the present invention, the filamentous phage is M13 and the vector additionally includes a restriction endonuclease site located in such a manner as to substantially inactivate the second gene X when a DNA sequence is inserted into the restriction site. 2 figures.

Inventors:
;
Publication Date:
OSTI Identifier:
6649482
Alternate Identifier(s):
OSTI ID: 6649482
Patent Number(s):
US 5376549; A
Application Number:
PPN: US 8-014944
Assignee:
Wisconsin Alumni Research Foundation, Madison, WI (United States) PTO; EDB-95-031423
DOE Contract Number:  
FG02-90ER61026
Resource Type:
Patent
Resource Relation:
Patent File Date: 5 Feb 1993
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; BACTERIOPHAGES; DNA SEQUENCING; DNA-CLONING; DNA REPAIR; ENDONUCLEASES; TRANSCRIPTION FACTORS; BIOLOGICAL RECOVERY; BIOLOGICAL REPAIR; BIOTECHNOLOGY; CLONING; DNA HYBRIDIZATION; DNA-ASE; ENZYMES; ESTERASES; GENETIC ENGINEERING; HYBRIDIZATION; HYDROLASES; MICROORGANISMS; NUCLEIC ACID HYBRIDIZATION; ORGANIC COMPOUNDS; PARASITES; PHOSPHODIESTERASES; PROTEINS; REPAIR; STRUCTURAL CHEMICAL ANALYSIS; VIRUSES 550200* -- Biochemistry

Citation Formats

Guilfoyle, R.A., and Smith, L.M. Cloning vector. United States: N. p., 1994. Web.
Guilfoyle, R.A., & Smith, L.M. Cloning vector. United States.
Guilfoyle, R.A., and Smith, L.M. Tue . "Cloning vector". United States.
@article{osti_6649482,
title = {Cloning vector},
author = {Guilfoyle, R.A. and Smith, L.M.},
abstractNote = {A vector comprising a filamentous phage sequence containing a first copy of filamentous phage gene X and other sequences necessary for the phage to propagate is disclosed. The vector also contains a second copy of filamentous phage gene X downstream from a promoter capable of promoting transcription in a bacterial host. In a preferred form of the present invention, the filamentous phage is M13 and the vector additionally includes a restriction endonuclease site located in such a manner as to substantially inactivate the second gene X when a DNA sequence is inserted into the restriction site. 2 figures.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1994},
month = {12}
}