Differentiation between endogenous ( sup 12 C) and applied ( sup 14 C or sup 13 C) IAA metabolism during maize root growth
- Institute of Biology and Plant Physiology of the Univ., Lausanne (Switzerland)
Intact primary maize (LG11) roots were immersed (1h) in a buffered solution containing IAA. Growth was measured 4h after treatment and three root zones (cap+apex, elongating, differentiating) were collected to determine the patterns of IAA metabolism (HPLC technique) and simultaneously level of both endogenous and applied IAA (GC-MS technique). By using HPLC separation and 2-{sup 14}C-IAA, different patterns of metabolism were identified for each root zone. The amount of labelled IAA depended upon the zone considered and the concentration used. Radioactivity was mainly distributed into two peaks: IAA and an unidentified polar substance. GC-MS technique was used quantify level of both endogenous {sup 12}C-IAA and applied {sup 13}C-IAA after immersion of 4h of growth. From data obtained for treated and untreated roots, the role of IAA metabolism during root growth is discussed.
- OSTI ID:
- 6649184
- Journal Information:
- Plant Physiology, Supplement; (USA), Vol. 89:4; ISSN 0079-2241
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
AUXINS
METABOLISM
MAIZE
PLANT GROWTH
ROOTS
CELL DIFFERENTIATION
CARBON 12
CARBON 13
CARBON 14 COMPOUNDS
GAS CHROMATOGRAPHY
MASS SPECTROSCOPY
TRACER TECHNIQUES
CARBON ISOTOPES
CEREALS
CHROMATOGRAPHY
EVEN-EVEN NUCLEI
EVEN-ODD NUCLEI
GRASS
GROWTH
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
LILIOPSIDA
MAGNOLIOPHYTA
NUCLEI
PLANT GROWTH REGULATORS
PLANTS
SEPARATION PROCESSES
SPECTROSCOPY
STABLE ISOTOPES
550501* - Metabolism- Tracer Techniques