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Title: Mineralization of sup 14 C-ring-labeled synthetic lignin correlates with the production of lignin peroxidase, not of manganese peroxidase or laccase. [Phanerochaete chrysosporium; Phlebia brevispora]

Abstract

Recently, Mn(II) has been shown to induce manganese peroxidases (MnPs) and repress lignin peroxidases (LiPs) in defined liquid cultures of several white rot organisms. The present work shows that laccase is also regulated by Mn(II). We therefore used Mn(II) to regulate production of LiP, MnP, and laccase activities while determining the effects of Mn(II) on mineralization of ring-labeled synthetic lignin. At a low Mn(II) level, Phanerochaete chrysosporium and Phlebia brevispora produced relatively high titers of LiPs but only low titers of MnPs. At a high Mn(II) level, MnP titers increased 12- to 20-fold, but LiPs were not detected in crude broths. P. brevispora formed much less LiP then P. chrysosporium, but it also produced laccase activity that increased more than sevenfold at the high Mn(II) level. The rates of synthetic lignin mineralization by these organisms were similar and were almost seven times higher at low than at high Mn(II). Increased synthetic lignin mineralization therefore correlated with increased LiP, not with increased MnP or laccase activities.

Authors:
;  [1]
  1. Department of Agriculture, Madison, WI (USA)
Publication Date:
OSTI Identifier:
6648826
Resource Type:
Journal Article
Journal Name:
Applied and Environmental Microbiology; (USA)
Additional Journal Information:
Journal Volume: 56:6; Journal ID: ISSN 0099-2240
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; LIGNIN; BIODEGRADATION; PEROXIDASES; ENZYME INDUCTION; CARBON 14 COMPOUNDS; ENZYME ACTIVITY; MINERALIZATION; SYNTHETIC MATERIALS; TRACER TECHNIQUES; CARBOHYDRATES; CHEMICAL REACTIONS; DECOMPOSITION; ENZYMES; GENE REGULATION; ISOTOPE APPLICATIONS; LABELLED COMPOUNDS; MATERIALS; ORGANIC COMPOUNDS; OXIDOREDUCTASES; POLYSACCHARIDES; SACCHARIDES; 090900* - Biomass Fuels- Processing- (1990-); 550201 - Biochemistry- Tracer Techniques

Citation Formats

Perez, J, and Jeffries, T W. Mineralization of sup 14 C-ring-labeled synthetic lignin correlates with the production of lignin peroxidase, not of manganese peroxidase or laccase. [Phanerochaete chrysosporium; Phlebia brevispora]. United States: N. p., 1990. Web.
Perez, J, & Jeffries, T W. Mineralization of sup 14 C-ring-labeled synthetic lignin correlates with the production of lignin peroxidase, not of manganese peroxidase or laccase. [Phanerochaete chrysosporium; Phlebia brevispora]. United States.
Perez, J, and Jeffries, T W. Fri . "Mineralization of sup 14 C-ring-labeled synthetic lignin correlates with the production of lignin peroxidase, not of manganese peroxidase or laccase. [Phanerochaete chrysosporium; Phlebia brevispora]". United States.
@article{osti_6648826,
title = {Mineralization of sup 14 C-ring-labeled synthetic lignin correlates with the production of lignin peroxidase, not of manganese peroxidase or laccase. [Phanerochaete chrysosporium; Phlebia brevispora]},
author = {Perez, J and Jeffries, T W},
abstractNote = {Recently, Mn(II) has been shown to induce manganese peroxidases (MnPs) and repress lignin peroxidases (LiPs) in defined liquid cultures of several white rot organisms. The present work shows that laccase is also regulated by Mn(II). We therefore used Mn(II) to regulate production of LiP, MnP, and laccase activities while determining the effects of Mn(II) on mineralization of ring-labeled synthetic lignin. At a low Mn(II) level, Phanerochaete chrysosporium and Phlebia brevispora produced relatively high titers of LiPs but only low titers of MnPs. At a high Mn(II) level, MnP titers increased 12- to 20-fold, but LiPs were not detected in crude broths. P. brevispora formed much less LiP then P. chrysosporium, but it also produced laccase activity that increased more than sevenfold at the high Mn(II) level. The rates of synthetic lignin mineralization by these organisms were similar and were almost seven times higher at low than at high Mn(II). Increased synthetic lignin mineralization therefore correlated with increased LiP, not with increased MnP or laccase activities.},
doi = {},
url = {https://www.osti.gov/biblio/6648826}, journal = {Applied and Environmental Microbiology; (USA)},
issn = {0099-2240},
number = ,
volume = 56:6,
place = {United States},
year = {1990},
month = {6}
}