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Title: Ammonium and nitrite inhibition of methane oxidation by methylobacter albus BG8 and methylosinus trichosporium OB3b at low methane concentrations

Abstract

Methane oxidation by pure cultures of the methanotrophs Methylobacter albus BG8 and Methylosinus trichosporium OB3b was inhibited by ammonium choride and sodium nitrite relative to that in cultures assayed in either nitrate-containing or nitrate-free medium. M. albus was generally more sensitive to ammonium and nitrate than M. Trichosporium. Both species produced nitrite from ammonium; the concentrations of nitrite produced increased with increasing methane concentrations in the culture headspaces. Inhibition of methane oxidation by nitrite was inversely proportional to headspace methane concentrations, with only minimal effects observed at concentrations of >500 ppm in the presence of 250 [mu]M nitrite. Inhibition increased with increasing ammonium at methane concentrations of 100 ppm. In the presence of 500 [mu]M ammonium, inhibition increased initially with increasing methane concentrations from 1.7 to 100 ppm; the extent of inhibition decreased with methane concentrations of >100 ppm. The results of this study provide new insights that explain some of the previously observed interactions among ammonium, nitrate, methane, and methane oxidation in soils and aquatic systems. 44 refs., 6 figs., 1 tab.

Authors:
;  [1]
  1. Univ. of Maine, Walpole, ME (United States)
Publication Date:
OSTI Identifier:
6648338
Resource Type:
Journal Article
Journal Name:
Applied and Environmental Microbiology; (United States)
Additional Journal Information:
Journal Volume: 60:10; Journal ID: ISSN 0099-2240
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 54 ENVIRONMENTAL SCIENCES; AMMONIUM CHLORIDES; ECOLOGICAL CONCENTRATION; CHEMICAL REACTIONS; INHIBITION; METHANE; OXIDATION; METHANOTROPHIC BACTERIA; BIOCHEMICAL REACTION KINETICS; SODIUM NITRATES; BIOLOGICAL PATHWAYS; SOILS; ALKALI METAL COMPOUNDS; ALKANES; AMMONIUM COMPOUNDS; AMMONIUM HALIDES; BACTERIA; CHLORIDES; CHLORINE COMPOUNDS; HALIDES; HALOGEN COMPOUNDS; HYDROCARBONS; KINETICS; MICROORGANISMS; NITRATES; NITROGEN COMPOUNDS; ORGANIC COMPOUNDS; OXYGEN COMPOUNDS; REACTION KINETICS; SODIUM COMPOUNDS; 550700* - Microbiology; 540220 - Environment, Terrestrial- Chemicals Monitoring & Transport- (1990-)

Citation Formats

King, G M, and Schnell, S. Ammonium and nitrite inhibition of methane oxidation by methylobacter albus BG8 and methylosinus trichosporium OB3b at low methane concentrations. United States: N. p., 1994. Web.
King, G M, & Schnell, S. Ammonium and nitrite inhibition of methane oxidation by methylobacter albus BG8 and methylosinus trichosporium OB3b at low methane concentrations. United States.
King, G M, and Schnell, S. Sat . "Ammonium and nitrite inhibition of methane oxidation by methylobacter albus BG8 and methylosinus trichosporium OB3b at low methane concentrations". United States.
@article{osti_6648338,
title = {Ammonium and nitrite inhibition of methane oxidation by methylobacter albus BG8 and methylosinus trichosporium OB3b at low methane concentrations},
author = {King, G M and Schnell, S},
abstractNote = {Methane oxidation by pure cultures of the methanotrophs Methylobacter albus BG8 and Methylosinus trichosporium OB3b was inhibited by ammonium choride and sodium nitrite relative to that in cultures assayed in either nitrate-containing or nitrate-free medium. M. albus was generally more sensitive to ammonium and nitrate than M. Trichosporium. Both species produced nitrite from ammonium; the concentrations of nitrite produced increased with increasing methane concentrations in the culture headspaces. Inhibition of methane oxidation by nitrite was inversely proportional to headspace methane concentrations, with only minimal effects observed at concentrations of >500 ppm in the presence of 250 [mu]M nitrite. Inhibition increased with increasing ammonium at methane concentrations of 100 ppm. In the presence of 500 [mu]M ammonium, inhibition increased initially with increasing methane concentrations from 1.7 to 100 ppm; the extent of inhibition decreased with methane concentrations of >100 ppm. The results of this study provide new insights that explain some of the previously observed interactions among ammonium, nitrate, methane, and methane oxidation in soils and aquatic systems. 44 refs., 6 figs., 1 tab.},
doi = {},
url = {https://www.osti.gov/biblio/6648338}, journal = {Applied and Environmental Microbiology; (United States)},
issn = {0099-2240},
number = ,
volume = 60:10,
place = {United States},
year = {1994},
month = {10}
}