Tumor affinity of radiolabeled peanut agglutinin compared with that of Ga-67 citrate in animal models
Peanut agglutinin (PNA) binds avidly to the immunodominant group of the tumor associated T antigen. The purpose of this study was to evaluate oncodiagnostic potential of radiolabeled PNA in animal models. PNA was labeled with I-125 or I-131 by Iodogen and also with In-111 by cyclic DTPA anhydride. The biological activity of PNA was examined by a hemaglutination titer with a photometer before and after labeling. Animal tumor models used were Lewis Lung Cancer(LLC), B-16 Melanotic Melanoma(MM), Yoshida Sarcoma(YS), Ehrlich Ascites Tumor(EAT and Hepatoma AH109A(HAH). Inflammatory tissue induced by turpentine oil was used as an abscess model. Serial scintigraphic images were obtained following IV injections of 100 ..mu..Ci of I-131 or In-111-DTPA-PNA. The tumor affinity of Ga-67 citrate was studied to compare that of radiolabeled PNA. Tissue biodistribution was studied in EAT bearing mice. All of these tumor models except HAH were clearly visible by radiolabeled PNA without subtraction techniques. In the models of LLC and EAT, PNA showed the better accumulation into the tumor tissue than Ga-67 citrate. In YS and MM, PNA represented almost the same accumulation as Ga-67 citrate. The localization of PNA into abscess tissue wasn't found although Ga-67 citrate markedly accumulated into abscess tissue as well as tumor tissue. The clearance of PNA from tumor was slower than those from any other organs. Tumor to muscle ratio was 5.1 at 48hrs. and tumor to blood ratio increased with time to 2.3 at 96hrs. These results suggested that radiolabeled PNA may have a potential in the detection of tumor.
- Research Organization:
- Kanazawa Univ. Hospital, Kanazawa
- OSTI ID:
- 6644223
- Report Number(s):
- CONF-850611-
- Journal Information:
- J. Nucl. Med.; (United States), Vol. 26:5; Conference: 32. annual meeting of the Society of Nuclear Medicine, Houston, TX, USA, 2 Jun 1985
- Country of Publication:
- United States
- Language:
- English
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AGGLUTININS
BINDING ENERGY
DIAGNOSTIC USES
LABELLING
PERFORMANCE
CITRATES
GALLIUM 67
IODINE 125
IODINE 131
NEOPLASMS
ANTIGENS
DIAGNOSIS
ANTIGEN-ANTIBODY REACTIONS
BIOLOGICAL ACCUMULATION
BIOLOGICAL LOCALIZATION
BLOOD
CLEARANCE
COMPARATIVE EVALUATIONS
INTRAVENOUS INJECTION
MICE
MUSCLES
PEANUTS
QUANTITY RATIO
RADIOIMMUNOASSAY
RADIONUCLIDE KINETICS
SCINTISCANNING
SIMULATION
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BODY FLUIDS
CARBOXYLIC ACID SALTS
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC TECHNIQUES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
ENERGY
GALLIUM ISOTOPES
IMMUNOASSAY
IMMUNOLOGY
INJECTION
INTAKE
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MATERIALS
NUCLEI
ODD-EVEN NUCLEI
RADIOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPE SCANNING
RADIOISOTOPES
RODENTS
SEEDS
TRACER TECHNIQUES
USES
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics