Site-specific cleavage of duplex DNA with a. lambda. repressor-staphylococcal nuclease hybrid
Journal Article
·
· Journal of the American Chemical Society; (USA)
- Univ. of California, Berkeley (USA)
The development of reagents capable of selective cleavage of large double-stranded DNA would greatly facilitate the manipulation and mapping of genomic DNA. We report here the design and synthesis of a {lambda} repressor-staphylococcal nuclease hybrid protein capable of efficient hydrolysis of both linear and supercoiled duplex DNA. The repressor domain of the hybrid nuclease binds specifically to its recognition sequence, {lambda} operator O{sub R}1 or O{sub L}1, and delivers the nuclease activity to DNA sequences adjacent to the repressor binding site.
- OSTI ID:
- 6644217
- Journal Information:
- Journal of the American Chemical Society; (USA), Vol. 112:11; ISSN 0002-7863
- Country of Publication:
- United States
- Language:
- English
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