Technique of neutron-induced (fission-track) autoradiography with histological detail

Journal Article · Mon Dec 01 00:00:00 EST 1980 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3575494· OSTI ID:6638041

Smith, J M; Taylor, G N; Jee, W S.S.

The primary advantage of neutron-induced or fission-track autoradiography compared with conventional autoradiography is that for low concentrations of fissile nuclides prohibitively long exposure times may be avoided. However, it is difficult to produce imaging of biological structures on the neutron-induced autoradiograph which would allow localization of the nuclide histologically. The technique presented circumvents this difficulty using a thin polycarbonate film applied to the histologically stained tissue section mounted on a quartz substrate. After irradiation of the tissue section with an appropriate thermal neutron flux, the fission fragment tracks are revealed by etching the film with KOH. The tracks, superimposed on the stained tissue, may be observed under the light microscope in the same manner as for conventional nuclear emulsion autoradiography.

• Research Organization: Univ. of Utah College of Medicine, Salt Lake City
• DOE Contract Number: EY-76-C-02-0119
• OSTI ID: 6638041
• Journal Information: Radiat. Res.; (United States), Vol. 84:3
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
FISSION TRACKS
AUTORADIOGRAPHY
LIVER
HISTOLOGY
OVARIES
PLUTONIUM 239
BEAGLES
CITRATES
ETCHING
PHOTOGRAPHIC FILMS
POLYCARBONATES
POTASSIUM HYDROXIDES
THERMAL NEUTRONS
ACTINIDE ISOTOPES
ACTINIDE NUCLEI
ALKALI METAL COMPOUNDS
ALPHA DECAY RADIOISOTOPES
ANIMALS
BARYONS
BODY
CARBON COMPOUNDS
CARBONATES
CARBOXYLIC ACID SALTS
DIGESTIVE SYSTEM
DOGS
ELEMENTARY PARTICLES
EVEN-ODD NUCLEI
FEMALE GENITALS
FERMIONS
GLANDS
GONADS
HADRONS
HEAVY NUCLEI
HYDROGEN COMPOUNDS
HYDROXIDES
ISOTOPES
MAMMALS
NEUTRONS
NUCLEI
NUCLEONS
ORGANS
OXYGEN COMPOUNDS
PARTICLE TRACKS
PLUTONIUM ISOTOPES
POTASSIUM COMPOUNDS
RADIOISOTOPES
SURFACE FINISHING
VERTEBRATES
YEARS LIVING RADIOISOTOPES
560162* - Radionuclide Effects
Kinetics
& Toxicology- Animals
Plants
Microorganisms
& Cells