Radioimmunochemical characterization of hemoglobins Lepore and Kenya: unique antigenic determinants located on hybrid hemoglobins. [Rabbits, /sup 125/I tracer technique]
Antisera were produced in rabbits to the three known types of Lepore hemoglobins, which contain hybrid delta-..beta.. non-..cap alpha..-chains, and to hemoglobin Kenya, which has a hybrid ..gamma..-..beta.. non-..cap alpha..-chain. By using a sensitive radioimmunoassay technique, the absorbed antisera were shown to contain an antibody population that was specific for the hybrid hemoglobin and did not cross-react with normal hemoglobins. However, with the absorbed Lepore-specific antisera, the three known types of Lepore hemoglobins were antigenically indistinguishable from each other, suggesting that antibodies are not produced to the primary structural differences which define the three non-..cap alpha..-chains of the Lepore hemoglobins. These studies demonstrate that the non-..cap alpha..-subunits of hemoglobins Lepore and Kenya possess unique antigenic determinant sites, evidently resulting from an altered polypeptide conformation.
- Research Organization:
- Medical Coll. of Georgia, Augusta
- OSTI ID:
- 6631457
- Journal Information:
- J. Immunol.; (United States), Vol. 121:R4
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
HEMOGLOBIN
HYBRIDIZATION
IMMUNE REACTIONS
RADIOIMMUNOASSAY
ANTIGENS
IMMUNE SERUMS
IODINE 125
RABBITS
TRACER TECHNIQUES
ANIMALS
BETA DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
GLOBIN
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PIGMENTS
PORPHYRINS
PROTEINS
RADIOASSAY
RADIOISOTOPES
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics