Sensitivity to UV radiation of small nuclear RNA synthesis in mammalian cells
It was demonstrated previously that the synthesis of small nuclear RNA (snRNA) species U1 and U2 in human cells is very sensitive to UV radiation. In the present work, the UV sensitivity of U3, U4, and U5 snRNA synthesis is shown to be also high. The synthesis of U1, U2, U3, U4, and U5 snRNAs progressively decreased during the first 2 h after UV irradiation (this was not observed in polyadenylated RNA) and had not returned to normal rates 6 h after UV exposure. In contrast, the restoration of 5.8S rRNA synthesis began immediately after UV irradiation and was essentially complete 6 h later. A small fraction of U1 and U5 (and possibly U2 and U3) snRNA synthesis remained unaffected by high UV doses, when cell radiolabeling began 10 min after UV irradiation. The present data suggest that a factor other than the level of pyrimidine dimers in DNA (possibly, steps in the post-irradiation DNA repair process) plays an important role in the mechanism of UV-induced inhibition of U1-U5 snRNA synthesis.
- Research Organization:
- St. Louis Univ. School of Medicine, MO
- OSTI ID:
- 6629544
- Journal Information:
- Mol. Cell. Biol.; (United States), Vol. 3:12
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
RNA
BIOSYNTHESIS
ULTRAVIOLET RADIATION
BIOLOGICAL EFFECTS
ANIMAL CELLS
BIOLOGICAL RECOVERY
CELL CULTURES
DOSE-RESPONSE RELATIONSHIPS
INHIBITION
TIME DEPENDENCE
ELECTROMAGNETIC RADIATION
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RADIATIONS
RECOVERY
SYNTHESIS
560111* - Radiation Effects on Biochemicals- In Vitro- (-1987)