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Title: Uptake of thymidine labeled on carbon 2: A potential index of liver regeneration by positron emission tomography

Abstract

Noninvasive measurement of liver regeneration with positron emission tomography has been attempted with 11C-thymidine; however, results were unsatisfactory using thymidine labeled on its methyl group. To evaluate whether the specificity of the method could be improved by modifying the labeling position of the tracer, thymidine labeled on its methyl group with 3H and thymidine labeled on its carbon 2 with 14C were injected in 22 hepatectomized rats either 1 hr (when DNA synthesis is not increased) or 24 hr after the surgical procedure (when the rate of DNA synthesis is maximal). Liver samples taken 10, 30 and 120 min after injection showed that, in contrast to 3H-radioactivity, 14C-radioactivity measured in whole tissue allowed a clear discrimination between regenerating and nonregenerating livers. In addition, 14C-radioactivity measured in whole tissue of regenerating livers correlated with the DNA radioactivity 10, 30 and 120 min after injection of the tracer. In contrast, no such correlation was found with the methyl-labeled thymidine. Analysis of the radioactive material present in the non-DNA fraction using ion exchange disks and high-performance liquid chromatography showed that 2-C-labeled thymidine was incorporated into DNA without accumulation of labeled metabolites whereas, for the methyl-labeled thymidine, almost all radioactivity was related to degradativemore » products. Therefore the evaluation of the liver regeneration with the 2-C-labeled thymidine, which does not require cellular fractionation, should be suited for noninvasive measurement with positron emission tomography.« less

Authors:
; ; ;  [1]
  1. Univ. of Louvain, Brussels (Belgium)
Publication Date:
OSTI Identifier:
6620168
Resource Type:
Journal Article
Journal Name:
Hepatology (Baltimore); (USA)
Additional Journal Information:
Journal Volume: 12:1; Journal ID: ISSN 0270-9139
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; LIVER; BIOLOGICAL REGENERATION; THYMIDINE; UPTAKE; CARBON 11; CARBON 14 COMPOUNDS; DNA REPLICATION; ION EXCHANGE; LIQUID COLUMN CHROMATOGRAPHY; POSITRON COMPUTED TOMOGRAPHY; RATS; REGRESSION ANALYSIS; TRACER TECHNIQUES; TRITIUM COMPOUNDS; ANIMALS; AZINES; BETA DECAY RADIOISOTOPES; BETA-PLUS DECAY RADIOISOTOPES; BIOLOGICAL RECOVERY; BODY; CARBON ISOTOPES; CHROMATOGRAPHY; COMPUTERIZED TOMOGRAPHY; DIAGNOSTIC TECHNIQUES; DIGESTIVE SYSTEM; EMISSION COMPUTED TOMOGRAPHY; EVEN-ODD NUCLEI; GLANDS; HETEROCYCLIC COMPOUNDS; HYDROGEN COMPOUNDS; ISOTOPE APPLICATIONS; ISOTOPES; LABELLED COMPOUNDS; LIGHT NUCLEI; MAMMALS; MATHEMATICS; MINUTES LIVING RADIOISOTOPES; NUCLEI; NUCLEIC ACID REPLICATION; NUCLEOSIDES; NUCLEOTIDES; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANS; PYRIMIDINES; RADIOISOTOPES; RECOVERY; RIBOSIDES; RODENTS; SEPARATION PROCESSES; STATISTICS; TOMOGRAPHY; VERTEBRATES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics

Citation Formats

Vander Borght, T M, Lambotte, L E, Pauwels, S A, and Dive, C C. Uptake of thymidine labeled on carbon 2: A potential index of liver regeneration by positron emission tomography. United States: N. p., 1990. Web. doi:10.1002/hep.1840120118.
Vander Borght, T M, Lambotte, L E, Pauwels, S A, & Dive, C C. Uptake of thymidine labeled on carbon 2: A potential index of liver regeneration by positron emission tomography. United States. https://doi.org/10.1002/hep.1840120118
Vander Borght, T M, Lambotte, L E, Pauwels, S A, and Dive, C C. 1990. "Uptake of thymidine labeled on carbon 2: A potential index of liver regeneration by positron emission tomography". United States. https://doi.org/10.1002/hep.1840120118.
@article{osti_6620168,
title = {Uptake of thymidine labeled on carbon 2: A potential index of liver regeneration by positron emission tomography},
author = {Vander Borght, T M and Lambotte, L E and Pauwels, S A and Dive, C C},
abstractNote = {Noninvasive measurement of liver regeneration with positron emission tomography has been attempted with 11C-thymidine; however, results were unsatisfactory using thymidine labeled on its methyl group. To evaluate whether the specificity of the method could be improved by modifying the labeling position of the tracer, thymidine labeled on its methyl group with 3H and thymidine labeled on its carbon 2 with 14C were injected in 22 hepatectomized rats either 1 hr (when DNA synthesis is not increased) or 24 hr after the surgical procedure (when the rate of DNA synthesis is maximal). Liver samples taken 10, 30 and 120 min after injection showed that, in contrast to 3H-radioactivity, 14C-radioactivity measured in whole tissue allowed a clear discrimination between regenerating and nonregenerating livers. In addition, 14C-radioactivity measured in whole tissue of regenerating livers correlated with the DNA radioactivity 10, 30 and 120 min after injection of the tracer. In contrast, no such correlation was found with the methyl-labeled thymidine. Analysis of the radioactive material present in the non-DNA fraction using ion exchange disks and high-performance liquid chromatography showed that 2-C-labeled thymidine was incorporated into DNA without accumulation of labeled metabolites whereas, for the methyl-labeled thymidine, almost all radioactivity was related to degradative products. Therefore the evaluation of the liver regeneration with the 2-C-labeled thymidine, which does not require cellular fractionation, should be suited for noninvasive measurement with positron emission tomography.},
doi = {10.1002/hep.1840120118},
url = {https://www.osti.gov/biblio/6620168}, journal = {Hepatology (Baltimore); (USA)},
issn = {0270-9139},
number = ,
volume = 12:1,
place = {United States},
year = {Sun Jul 01 00:00:00 EDT 1990},
month = {Sun Jul 01 00:00:00 EDT 1990}
}