Removal of phenols and aromatic amines from wastewater by a combination treatment with tyrosinase and a coagulant

Journal Article · Mon Feb 20 00:00:00 EST 1995 · Biotechnology and Bioengineering; (United States)

DOI:https://doi.org/10.1002/bit.260450404· OSTI ID:6619897

Wada, Shinji; Ichikawa, Hiroyasu; Tatsumi, Kenji ^[1]

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1. National Inst. for Resources and Environment, Ibaraki (Japan)

Removal of phenols and aromatic amines from industrial wastewater by tyrosinase was investigated. A color change from colorless to dark brown was observed, but no precipitate was formed. Colored products were found to be easily removed by a combination treatment with tyrosinase and a cationic polymer coagulant containing amino group, such as hexamethylenediamine-epichlorohidrin polycondensate, polyethleneimine, or chitosan. The first two coagulants, synthetic polymers, were more effective than chitosan, a polymer produced in crustacean shells. Phenols and aromatic amines are not precipitated by any kind of coagulants, but their enzymatic reaction products are easily precipitated by a cationic polymer coagulant. These results indicate that the combination of tyrosinase and a cationic polymer coagulant is effective in removing carcinogenic phenols and aromatic amines from an aqueous solution. Immobilization of tyrosinase on magnetite gave a good retention of activity (80%) and storage stability i.e., only 5% loss after 15 days of storage at ambient temperature. In the treatment of immobilized tyrosinase, colored enzymatic reaction products were removed by less coagulant compared with soluble tyrosinase.

• OSTI ID: 6619897
• Journal Information: Biotechnology and Bioengineering; (United States), Vol. 45:4; ISSN 0006-3592
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
AMINES
REMOVAL
AROMATICS
COAGULANTS
SORPTIVE PROPERTIES
PHENOLS
TYROSINASE
ENZYME ACTIVITY
WASTE WATER
DECONTAMINATION
IMMOBILIZED ENZYMES
INDUSTRIAL WASTES
CLEANING
DRUGS
ENZYMES
HEMATOLOGIC AGENTS
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
HYDROXYLASES
LIQUID WASTES
ORGANIC COMPOUNDS
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PROTEINS
SURFACE PROPERTIES
WASTES
WATER
540320* - Environment
Aquatic- Chemicals Monitoring & Transport- (1990-)