Chromosomal reassignment: YACs containing both YES1 and thymidylate synthase map to the short arm of chromosome 18

Journal Article · Mon Feb 01 00:00:00 EST 1993 · Genomics; (United States)

DOI:https://doi.org/10.1006/geno.1993.1086· OSTI ID:6619478

Silverman, G A ^[1]; Kuo, Wenlin; Gray, J W ^[2]; Taillon-Miller, P ^[3]

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1. Harvard Medical School, Boston, MA (United States)
2. Univ. of California, San Francisco (United States)
3. Washington Univ. School of Medicine, St. Louis, MO (United States)

The YES1 proto-oncogene was mapped previously to human chromosome band 18q21.3 by using isotopic in situ hybridization. Using yeast artificial chromosomes (YACs) as probes and fluorescence in situ hybridization, a strong signal was detected in the region corresponding to 18p11.3. Restriction digests confirmed that the YACs contained the YES1 gene and not other cross-hybridizing, protein-tyrosine kinases. In addition, these YACs were found to contain another 18p11.32 gene, thymidylate synthase. These genes were less than 50 kb apart. Collectively, these data suggest that YES1 maps to 18p11.32 rather than to 18p21.3. 18 refs., 3 figs.

• OSTI ID: 6619478
• Journal Information: Genomics; (United States), Vol. 15:2; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
HUMAN CHROMOSOME 18
GENETIC MAPPING
ONCOGENES
CHROMOSOMES
ENZYMES
FLUORESCENCE
GENES
HYBRIDIZATION
MAPS
PROBES
SYNTHESIS
THYMIDYLIC ACID
HUMAN CHROMOSOMES
LUMINESCENCE
MAPPING
NUCLEOTIDES
ORGANIC COMPOUNDS
PROTEINS
550400* - Genetics