skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Isolation of a novel human papillomavirus (type 51) from a cervical condyloma

Abstract

The authors cloned the DNA from a novel human papillomavirus (HPV) present in a cervical condyloma. When DNA from this isolate was hybridized at high stringency with HPV types 1 through 50 (HPV-1 through HPV-50), it showed weak homology with HPV-6 and -16 and stronger homology with HPV-26. A detailed restriction endonuclease map was prepared which showed marked differences from the maps for other HPVs that have been isolated from the female genital tract. Reassociation kinetic analysis revealed that HPV-26 and this new isolate were less than 10% homologous; hence, the new isolate is a noel strain of HPV. The approximate positions of the open reading frames of the new strain were surmised by hybridization with probes derived from individual open reading frames of HPV-16. In an analysis of 175 genital biopsies from patients with abnormal Papanicolaou smears, sequences hybridizing under highly stringent conditions to probes from this novel HPV type were found in 4.2, 6.1, and 2.4% of biopsies containing normal squamous epithelium, condylomata, and intraepithelial neoplasia, respectively. In addition, sequences homologous to probes from this novel isolate were detected in one of five cervical carcinomas examined.

Authors:
; ; ;  [1];  [2]
  1. (Columbia Univ., New York, NY (USA))
  2. (Deutsches Krebsforschungszentrum, Heidelberg (West Germany))
Publication Date:
OSTI Identifier:
6617502
Alternate Identifier(s):
OSTI ID: 6617502
Resource Type:
Journal Article
Resource Relation:
Journal Name: Journal of Virology; (USA); Journal Volume: 62:4
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; TUMOR CELLS; DNA SEQUENCING; UTERUS; CARCINOMAS; VIRUSES; DNA-CLONING; BIOPSY; DNA HYBRIDIZATION; EPITHELIUM; PHOSPHORUS 32; UROGENITAL SYSTEM DISEASES; WOMEN; ANIMAL CELLS; ANIMAL TISSUES; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BODY; CLONING; DAYS LIVING RADIOISOTOPES; DIAGNOSTIC TECHNIQUES; DISEASES; FEMALE GENITALS; FEMALES; HYBRIDIZATION; ISOTOPES; LIGHT NUCLEI; MAMMALS; MAN; MICROORGANISMS; NEOPLASMS; NUCLEI; ODD-ODD NUCLEI; ORGANS; PARASITES; PHOSPHORUS ISOTOPES; PRIMATES; RADIOISOTOPES; STRUCTURAL CHEMICAL ANALYSIS; TISSUES; VERTEBRATES 550701* -- Microbiology-- Tracer Techniques

Citation Formats

Nuovo, G.J., Crum, C.P., Levine, R.U., Silverstein, S.J., and De Villiers, E.M. Isolation of a novel human papillomavirus (type 51) from a cervical condyloma. United States: N. p., 1988. Web.
Nuovo, G.J., Crum, C.P., Levine, R.U., Silverstein, S.J., & De Villiers, E.M. Isolation of a novel human papillomavirus (type 51) from a cervical condyloma. United States.
Nuovo, G.J., Crum, C.P., Levine, R.U., Silverstein, S.J., and De Villiers, E.M. Fri . "Isolation of a novel human papillomavirus (type 51) from a cervical condyloma". United States.
@article{osti_6617502,
title = {Isolation of a novel human papillomavirus (type 51) from a cervical condyloma},
author = {Nuovo, G.J. and Crum, C.P. and Levine, R.U. and Silverstein, S.J. and De Villiers, E.M.},
abstractNote = {The authors cloned the DNA from a novel human papillomavirus (HPV) present in a cervical condyloma. When DNA from this isolate was hybridized at high stringency with HPV types 1 through 50 (HPV-1 through HPV-50), it showed weak homology with HPV-6 and -16 and stronger homology with HPV-26. A detailed restriction endonuclease map was prepared which showed marked differences from the maps for other HPVs that have been isolated from the female genital tract. Reassociation kinetic analysis revealed that HPV-26 and this new isolate were less than 10% homologous; hence, the new isolate is a noel strain of HPV. The approximate positions of the open reading frames of the new strain were surmised by hybridization with probes derived from individual open reading frames of HPV-16. In an analysis of 175 genital biopsies from patients with abnormal Papanicolaou smears, sequences hybridizing under highly stringent conditions to probes from this novel HPV type were found in 4.2, 6.1, and 2.4% of biopsies containing normal squamous epithelium, condylomata, and intraepithelial neoplasia, respectively. In addition, sequences homologous to probes from this novel isolate were detected in one of five cervical carcinomas examined.},
doi = {},
journal = {Journal of Virology; (USA)},
number = ,
volume = 62:4,
place = {United States},
year = {Fri Apr 01 00:00:00 EST 1988},
month = {Fri Apr 01 00:00:00 EST 1988}
}