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Title: Identification of the transcriptional unit, structural organization, and promoter sequence of the human sex-determining region Y (SRY) gene, using a reverse genetic approach

Abstract

Using a simple strategy involving cosmid-mediated gene transfer, cDNA library construction, and molecular characterization techniques, the authors have determined the transcriptional unit, structural organization, and promoter sequence of the human sex-determining region Y (SRY) gene, the putative testis-determining factor (TDF) gene on the human Y chromosome. By this approach, a recombinant cosmid harboring the human SRY sequence was isolated and transfected to appropriate tissue-cultured cells. Recombinant cDNA clones were isolated from a cDNA library constructed from poly (A) + RNA of the transfected cells. Comparative studies between the respective cDNAs and the genomic cosmid have provided information regarding the organization of the SRY gene and its mRNAs. The results indicate that the human SRY gene is an intronless gene, produces transcripts of 1.1 kb, and possesses promoter activities in the transfected cells at approximately 310 bp of its upstream sequences. 57 refs., 5 figs.

Authors:
;  [1]
  1. Univ. of California, San Francisco (United States)
Publication Date:
OSTI Identifier:
6616131
Resource Type:
Journal Article
Journal Name:
American Journal of Human Genetics; (United States)
Additional Journal Information:
Journal Volume: 52:1; Journal ID: ISSN 0002-9297
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; HUMAN Y CHROMOSOME; GENES; SEX; GENE REPRESSORS; CONSTRUCTION; COSMIDS; DNA-CLONING; LIBRARIES; RNA; CHROMOSOMES; CLONING; DNA HYBRIDIZATION; HETEROCHROMOSOMES; HUMAN CHROMOSOMES; HYBRIDIZATION; NUCLEIC ACIDS; ORGANIC COMPOUNDS; Y CHROMOSOME; 550400* - Genetics

Citation Formats

Su, Hua, and Lau, Y F.C. Identification of the transcriptional unit, structural organization, and promoter sequence of the human sex-determining region Y (SRY) gene, using a reverse genetic approach. United States: N. p., 1993. Web.
Su, Hua, & Lau, Y F.C. Identification of the transcriptional unit, structural organization, and promoter sequence of the human sex-determining region Y (SRY) gene, using a reverse genetic approach. United States.
Su, Hua, and Lau, Y F.C. 1993. "Identification of the transcriptional unit, structural organization, and promoter sequence of the human sex-determining region Y (SRY) gene, using a reverse genetic approach". United States.
@article{osti_6616131,
title = {Identification of the transcriptional unit, structural organization, and promoter sequence of the human sex-determining region Y (SRY) gene, using a reverse genetic approach},
author = {Su, Hua and Lau, Y F.C.},
abstractNote = {Using a simple strategy involving cosmid-mediated gene transfer, cDNA library construction, and molecular characterization techniques, the authors have determined the transcriptional unit, structural organization, and promoter sequence of the human sex-determining region Y (SRY) gene, the putative testis-determining factor (TDF) gene on the human Y chromosome. By this approach, a recombinant cosmid harboring the human SRY sequence was isolated and transfected to appropriate tissue-cultured cells. Recombinant cDNA clones were isolated from a cDNA library constructed from poly (A) + RNA of the transfected cells. Comparative studies between the respective cDNAs and the genomic cosmid have provided information regarding the organization of the SRY gene and its mRNAs. The results indicate that the human SRY gene is an intronless gene, produces transcripts of 1.1 kb, and possesses promoter activities in the transfected cells at approximately 310 bp of its upstream sequences. 57 refs., 5 figs.},
doi = {},
url = {https://www.osti.gov/biblio/6616131}, journal = {American Journal of Human Genetics; (United States)},
issn = {0002-9297},
number = ,
volume = 52:1,
place = {United States},
year = {1993},
month = {1}
}