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Title: Effect of nutrient limitation on genomic rearrangements in prokaryotes. Final report

Abstract

During the total agreement period, more than 200 unique heterotrophic aerobic bacteria were isolated from samples of deep subsurface sediments retrieved from depths of 149.2 and 178.9 m of a vertical bore hole at Cerro Negro (CNV1). All the isolates were characterized phylogenetically and deposited to SMCC. Several clones belonging to a family currently believed to be of strictly marine origin (Microscilla) were found among the isolates. The bacteria might be the descendants of those present in marine environment at the time of deposition. This is an important conclusion for the entire deep subsurface Microbial Origins project. The authors determined diversity of populations of prokaryotic genomes of independent bacterial clones isolated from both surface soil, and subsurface sediments. The degree of diversity for deep subsurface isolates was exceedingly high, much higher than that observed for surface soil isolates. The authors have found that the genomes of some subsurface isolates are significantly more plastic than those for strains isolated from surface environments. The most intriguing discovery was isolation and cultivation of populations of nonplateable novel ultra small bacteria, which are phylogenetically more close to chloroplasts of higher plants, than any other existing bacteria including Cyanobacteria.

Authors:
;
Publication Date:
Research Org.:
NSF Center for Microbial Ecology, East Lansing, MI (United States); Michigan State Univ., East Lansing, MI (United States)
Sponsoring Org.:
USDOE Office of Energy Research, Washington, DC (United States)
OSTI Identifier:
661515
Report Number(s):
DOE/ER/61681-T1
ON: DE98007202; TRN: AHC29814%%94
DOE Contract Number:
FG02-93ER61681
Resource Type:
Technical Report
Resource Relation:
Other Information: PBD: Mar 1998
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; PROGRESS REPORT; BACTERIA; SPECIES DIVERSITY; NUTRIENTS; GROWTH; SEDIMENTS; GENETIC VARIABILITY; DNA-CLONING; KARYOTYPE; PHENOTYPE

Citation Formats

Zlatkin, I.V., and Forney, L.J. Effect of nutrient limitation on genomic rearrangements in prokaryotes. Final report. United States: N. p., 1998. Web. doi:10.2172/661515.
Zlatkin, I.V., & Forney, L.J. Effect of nutrient limitation on genomic rearrangements in prokaryotes. Final report. United States. doi:10.2172/661515.
Zlatkin, I.V., and Forney, L.J. Sun . "Effect of nutrient limitation on genomic rearrangements in prokaryotes. Final report". United States. doi:10.2172/661515. https://www.osti.gov/servlets/purl/661515.
@article{osti_661515,
title = {Effect of nutrient limitation on genomic rearrangements in prokaryotes. Final report},
author = {Zlatkin, I.V. and Forney, L.J.},
abstractNote = {During the total agreement period, more than 200 unique heterotrophic aerobic bacteria were isolated from samples of deep subsurface sediments retrieved from depths of 149.2 and 178.9 m of a vertical bore hole at Cerro Negro (CNV1). All the isolates were characterized phylogenetically and deposited to SMCC. Several clones belonging to a family currently believed to be of strictly marine origin (Microscilla) were found among the isolates. The bacteria might be the descendants of those present in marine environment at the time of deposition. This is an important conclusion for the entire deep subsurface Microbial Origins project. The authors determined diversity of populations of prokaryotic genomes of independent bacterial clones isolated from both surface soil, and subsurface sediments. The degree of diversity for deep subsurface isolates was exceedingly high, much higher than that observed for surface soil isolates. The authors have found that the genomes of some subsurface isolates are significantly more plastic than those for strains isolated from surface environments. The most intriguing discovery was isolation and cultivation of populations of nonplateable novel ultra small bacteria, which are phylogenetically more close to chloroplasts of higher plants, than any other existing bacteria including Cyanobacteria.},
doi = {10.2172/661515},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Sun Mar 01 00:00:00 EST 1998},
month = {Sun Mar 01 00:00:00 EST 1998}
}

Technical Report:

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