UDP-( sup 14 C)glucose-labelable polypeptides from pea: Possible components of glucan synthase I activity
- Stanford Univ., CA (USA)
A membrane-bound polypeptide doublet of about 40 kD can be rapidly labeled with UDP-({sup 14}C)glucose under the assay conditions for glucan synthase I (GS-I). Label seems covalently bound, and chases when unlabeled UDPG is added; it might represent a covalent intermediate in polysaccharide synthesis. Labeling and GS-I activity show several common features: they co-sediment with Golgi membranes in sucrose gradients; they depend similarly on Mg{sup 2+} or Mn{sup 2+} (not Ca{sup 2+}); they decrease dramatically from stem apex to base, and are higher in epidermis than internal tissue; they show similar sensitivities to several inhibitors. But the doublet still labels after polysaccharide-synthesizing activity has been destroyed by Triton X-100. The doublet polypeptides might be glucosyl tranferases whose ability to transfer glucose units to a glucan chain is detergent-sensitive, but to accept glucose from UDPG is not; or they might be detergent-insensitive primary glucose acceptors, from which a distinct, detergent-sensitive transferase(s) move(s) these units to glucan chains.
- OSTI ID:
- 6610847
- Journal Information:
- Plant Physiology, Supplement; (USA), Vol. 89:4; ISSN 0079-2241
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
POLYPEPTIDES
LABELLING
TRANSFERASES
ENZYME ACTIVITY
CARBON 14 COMPOUNDS
CELL MEMBRANES
GLUCOSE
MEMBRANE PROTEINS
TRACER TECHNIQUES
ALDEHYDES
CARBOHYDRATES
CELL CONSTITUENTS
ENZYMES
HEXOSES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MEMBRANES
MONOSACCHARIDES
ORGANIC COMPOUNDS
PEPTIDES
PROTEINS
SACCHARIDES
550201* - Biochemistry- Tracer Techniques