Influence of dietary cholesterol on 26-hydroxycholesterol and the effect of 26-hydroxycholesterol on the intracellular free calcium level

Thesis/Dissertation · Thu Jan 01 00:00:00 EST 1987

OSTI ID:6602784

Kou, I L

The purpose of this study was to investigate the factors influencing serum level of 26-hydroxycholesterol after long-term consumption of cholesterol by animals. It is also to examine the effect of this sterol on intracellular free calcium level. Purified 26-hydroxycholesterol was synthesized from kryptogenin by the Clemmemsen and Wolff-Kishner reduction method. 26-Hydroxycholesterol was also used for fatty acid esters syntheses, and to study its influence on membranes. Tritiated 26-hydroxycholesterol which was synthesized by an enzymatic method, was used to monitor the 26-hydroxycholesterol loss during the procedure. The ester form of 26-hydroxycholesterol was also synthesized, and used to investigate its effects on membranes. The HPLC method that was developed for the analysis of 26-hydroxycholesterol levels in animal tissues was accurate, efficient, and reproducible for the determination of 26-hydroxycholesterol in plasma. However, it was not suitable for the analysis of other tissues, due to the overlapping of peaks making quantitation difficult.

• Research Organization: Illinois Univ., Urbana (USA)
• OSTI ID: 6602784
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CALCIUM
BIOSYNTHESIS
CHOLESTEROL
BIOLOGICAL EFFECTS
CARBOXYLIC ACIDS
CELL MEMBRANES
ESTERS
LIQUID COLUMN CHROMATOGRAPHY
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ALKALINE EARTH METALS
CELL CONSTITUENTS
CHROMATOGRAPHY
ELEMENTS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MEMBRANES
METALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
SEPARATION PROCESSES
STEROIDS
STEROLS
SYNTHESIS
550201* - Biochemistry- Tracer Techniques