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Title: Radiosensitivity and thermosensitization of thermotolerant Chinese hamster cells and RIF-1 tumors

Abstract

CHO cells subline HA-1 were made thermotolerant by a priming heat treatment(43/sup 0/C, 30 min). Later, 4, 16, or 24 hr, they were either irradiated or heated (43/sup 0/C, 30 min) and irradiated. Thermotolerance had no effect on the radiation sensitivity of the cells as measured by the D/sub 0/ value of the clonogenic survival curve. However the N value of the curve (width of shoulder) showed a significant increase at 24 hr, indicating an increased capacity to accumulate sublethal damage. The same priming treatment was given to RIF-1 tumors growing in C3H mice. Later, 24 hr, when the tumors were either irradiated or heated (43/sup 0/C, 30 min) and irradiated, it was found that thermotolerance had no effect on the radiosensitivity of the cells as measured by in vitro assay. However, thermal radiosensitization was not apparent 24 hr after the priming treatment.

Authors:
; ;
Publication Date:
Research Org.:
Vanderbilt Univ. Hospital, Nashville, TN
OSTI Identifier:
6600802
Resource Type:
Journal Article
Journal Name:
Radiat. Res.; (United States)
Additional Journal Information:
Journal Volume: 99:1
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; CELL KILLING; RADIOINDUCTION; CHO CELLS; BIOLOGICAL RADIATION EFFECTS; TEMPERATURE EFFECTS; EXPERIMENTAL NEOPLASMS; HYPERTHERMIA; RADIOSENSITIVITY EFFECTS; MICE; STATISTICS; SURVIVAL CURVES; X RADIATION; ANIMAL CELLS; ANIMALS; BIOLOGICAL EFFECTS; BODY TEMPERATURE; ELECTROMAGNETIC RADIATION; IONIZING RADIATIONS; MAMMALS; MATHEMATICS; RADIATION EFFECTS; RADIATIONS; RODENTS; VERTEBRATES; 560121* - Radiation Effects on Cells- External Source- (-1987); 560201 - Thermal Effects- Cells- (-1987)

Citation Formats

Hartson-Eaton, M, Malcolm, A W, and Hahn, G M. Radiosensitivity and thermosensitization of thermotolerant Chinese hamster cells and RIF-1 tumors. United States: N. p., 1984. Web. doi:10.2307/3576455.
Hartson-Eaton, M, Malcolm, A W, & Hahn, G M. Radiosensitivity and thermosensitization of thermotolerant Chinese hamster cells and RIF-1 tumors. United States. https://doi.org/10.2307/3576455
Hartson-Eaton, M, Malcolm, A W, and Hahn, G M. 1984. "Radiosensitivity and thermosensitization of thermotolerant Chinese hamster cells and RIF-1 tumors". United States. https://doi.org/10.2307/3576455.
@article{osti_6600802,
title = {Radiosensitivity and thermosensitization of thermotolerant Chinese hamster cells and RIF-1 tumors},
author = {Hartson-Eaton, M and Malcolm, A W and Hahn, G M},
abstractNote = {CHO cells subline HA-1 were made thermotolerant by a priming heat treatment(43/sup 0/C, 30 min). Later, 4, 16, or 24 hr, they were either irradiated or heated (43/sup 0/C, 30 min) and irradiated. Thermotolerance had no effect on the radiation sensitivity of the cells as measured by the D/sub 0/ value of the clonogenic survival curve. However the N value of the curve (width of shoulder) showed a significant increase at 24 hr, indicating an increased capacity to accumulate sublethal damage. The same priming treatment was given to RIF-1 tumors growing in C3H mice. Later, 24 hr, when the tumors were either irradiated or heated (43/sup 0/C, 30 min) and irradiated, it was found that thermotolerance had no effect on the radiosensitivity of the cells as measured by in vitro assay. However, thermal radiosensitization was not apparent 24 hr after the priming treatment.},
doi = {10.2307/3576455},
url = {https://www.osti.gov/biblio/6600802}, journal = {Radiat. Res.; (United States)},
number = ,
volume = 99:1,
place = {United States},
year = {Sun Jul 01 00:00:00 EDT 1984},
month = {Sun Jul 01 00:00:00 EDT 1984}
}