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Title: Combined capillary electrophoresis and electrospray ionization mass spectrometry

Abstract

The development of new capillary electrophoresis (CE) methods provides a basis for the efficient manipulation and separation of subpicomole quantities of polypeptides and proteins. Recent advances in microscale methods, such as the demonstration of the tryptic digestion of low picomole quantities of proteins using the immobilized enzyme in small diameter packed reactor column, provide the basis for such further developments. The use of capillary (free solution) zone electrophoresis (CZE) for separation of proteins, and recent demonstrations of restriction mapping of large deoxyribonucleotides, has propelled potential CE applications into the realm of conventional electrophoresis, while adding the attributes of speed, relatively simple on-line detection, automation, and reduced sample requirements (10{sup {minus}17} {minus} 10 {sup {minus}13} mole). A literal explosion of ancillary methods for sample manipulation, derivatization, and detection as well as new methods of obtaining separation selectivity are being reported. Additionally, other CE formats are attracting increased interest, with the aim of exploiting the unique features of capillary isotachophoresis (CITP), capillary isoelectric focusing (CIEF), capillary electrokinetic chromatograpgy (CEC), and most recently, capillary polyacrylamide gel electrophoresis (CGE). As a result, there are concomitant and increasing demands upon detector sensitivity and information density.

Authors:
; ; ;
Publication Date:
Research Org.:
Pacific Northwest Lab., Richland, WA (USA)
Sponsoring Org.:
DOE/ER; National Science Foundation (NSF)
OSTI Identifier:
6594739
Report Number(s):
PNL-SA-18081; CONF-9007162-1
ON: DE90016535
DOE Contract Number:  
AC06-76RL01830
Resource Type:
Conference
Resource Relation:
Conference: Spectroscopy across the spectra, Hertford (UK), 9-12 Jul 1990
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; 59 BASIC BIOLOGICAL SCIENCES; POLYPEPTIDES; SEPARATION PROCESSES; PROTEINS; ELECTROPHORESIS; IONIZATION; MASS SPECTROSCOPY; POLYMERS; ORGANIC COMPOUNDS; PEPTIDES; SPECTROSCOPY; 400105* - Separation Procedures; 400102 - Chemical & Spectral Procedures; 550200 - Biochemistry

Citation Formats

Smith, R D, Loo, J A, Edmonds, C G, and Udseth, H R. Combined capillary electrophoresis and electrospray ionization mass spectrometry. United States: N. p., 1990. Web.
Smith, R D, Loo, J A, Edmonds, C G, & Udseth, H R. Combined capillary electrophoresis and electrospray ionization mass spectrometry. United States.
Smith, R D, Loo, J A, Edmonds, C G, and Udseth, H R. Sun . "Combined capillary electrophoresis and electrospray ionization mass spectrometry". United States.
@article{osti_6594739,
title = {Combined capillary electrophoresis and electrospray ionization mass spectrometry},
author = {Smith, R D and Loo, J A and Edmonds, C G and Udseth, H R},
abstractNote = {The development of new capillary electrophoresis (CE) methods provides a basis for the efficient manipulation and separation of subpicomole quantities of polypeptides and proteins. Recent advances in microscale methods, such as the demonstration of the tryptic digestion of low picomole quantities of proteins using the immobilized enzyme in small diameter packed reactor column, provide the basis for such further developments. The use of capillary (free solution) zone electrophoresis (CZE) for separation of proteins, and recent demonstrations of restriction mapping of large deoxyribonucleotides, has propelled potential CE applications into the realm of conventional electrophoresis, while adding the attributes of speed, relatively simple on-line detection, automation, and reduced sample requirements (10{sup {minus}17} {minus} 10 {sup {minus}13} mole). A literal explosion of ancillary methods for sample manipulation, derivatization, and detection as well as new methods of obtaining separation selectivity are being reported. Additionally, other CE formats are attracting increased interest, with the aim of exploiting the unique features of capillary isotachophoresis (CITP), capillary isoelectric focusing (CIEF), capillary electrokinetic chromatograpgy (CEC), and most recently, capillary polyacrylamide gel electrophoresis (CGE). As a result, there are concomitant and increasing demands upon detector sensitivity and information density.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1990},
month = {7}
}

Conference:
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