Biochemical, biophysical, and molecular genetic studies on the membrane-bound D-lactate dehydrogenase from Escherichia coli

Thesis/Dissertation · Wed Jan 01 00:00:00 EST 1986

OSTI ID:6592679

Rule, G S

The membrane-bound D-lactate dehydrogenase (D-LDH) from E. coli has been used as a model system in order to study structure-function relationships in membrane proteins. This enzyme is activated by various lipids and detergents in vitro, and appears to provide energy for the active transport of amino acids and sugars in E. coli membrane vesicles. In order to obtain sufficient amounts of enzyme for /sup 19/F nuclear magnetic resonance (NMR) and circular dichroism (CD) experiments, the cloned dld gene was used to construct a plasmid in which the expression of D-LDH is induced by a temperature shift. Upon temperature induction, the presence of this plasmid results in levels of D-LDH in the cell which are 300-fold higher than wild type levels. The cloned dld gene was also sequenced and the primary structure of D-LDH, as deduced from the DNA sequence, was verified by determination of the amino-terminal sequence and the amino acid composition of D-LDH. The dld gene codes for a protein which does not contain a signal sequence and is 571 residues long.

• Research Organization: Carnegie-Mellon Univ., Pittsburgh, PA (USA)
• OSTI ID: 6592679
• Resource Relation: Other Information: Thesis (Ph. D)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GENES
DNA SEQUENCING
OXIDOREDUCTASES
STRUCTURE-ACTIVITY RELATIONSHIPS
AMINO ACID SEQUENCE
BIOLOGICAL MODELS
ESCHERICHIA COLI
FLUORINE 19
MOLECULAR STRUCTURE
NMR SPECTRA
NUCLEAR MAGNETIC RESONANCE
BACTERIA
ENZYMES
FLUORINE ISOTOPES
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
MICROORGANISMS
NUCLEI
ODD-EVEN NUCLEI
RESONANCE
SPECTRA
STABLE ISOTOPES
STRUCTURAL CHEMICAL ANALYSIS
550200* - Biochemistry