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Title: Environmental restoration using plant-microbe bioaugmentation

Abstract

Land farming, for the purpose of bioremediation, refers traditionally to the spreading of contaminated soil, sediments, or other material over land; mechanically mixing it; incorporating various amendments, such as fertilizer or mulch; and sometimes inoculating with degradative microorganisms. Populations of bacteria added to soils often decline rapidly and become metabolically inactive. To efficiently degrade contaminants, microorganisms must be metabolically active. Thus, a significant obstacle to the successful use of microorganisms for environmental applications is their long-term survival and the expression of their degradative genes in situ. Rhizosphere microorganisms are known to be more metabolically active than those in bulk soil, because they obtain carbon and energy from root exudates and decaying root matter. Rhizosphere populations are also more abundant, often containing 10[sup 8] or more culturable bacteria per gram of soil, and bacterial populations on the rhizoplane can exceed 10[sup 9]/g root. Many of the critical parameters that influence the competitive ability of rhizosphere bacteria have not been identified, but microorganisms have frequently been introduced into soil (bioaugmentation) as part of routine or novel agronomic practices. However, the use of rhizosphere bacteria and their in situ stimulation by plant roots for degrading organic contaminants has received little attention. Published studiesmore » have demonstrated the feasibility of using rhizobacteria (Pseudomonas putida) for the rapid removal of chlorinated pesticides from contaminated soil, and to promote germination of radish seeds in the presence of otherwise phytotoxic levels of the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D), and phenoxyacetic acid (PAA). The present investigation was undertaken to determine if these strains (Pseudomonas putida PP0301/pRO101 and PP0301/pRO103) could be used to bioremediate 2,4-D-amended soil via plant-microbe bioaugmentation.« less

Authors:
; ; ;
Publication Date:
Research Org.:
Pacific Northwest Lab., Richland, WA (United States)
Sponsoring Org.:
USDOE; USDOE, Washington, DC (United States)
OSTI Identifier:
6591767
Report Number(s):
PNL-SA-22251; CONF-930482-6
ON: DE93013160
DOE Contract Number:  
AC06-76RL01830
Resource Type:
Conference
Resource Relation:
Conference: 2. international symposium in situ and on-site bioreclamation, San Diego, CA (United States), 5-8 Apr 1993
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; 59 BASIC BIOLOGICAL SCIENCES; BIODEGRADATION; AUGMENTATION; LAND RECLAMATION; OPTIMIZATION; MICROORGANISMS; SURVIVAL TIME; PLANT GROWTH; BACTERIA; CARBON DIOXIDE FIXATION; NITROGEN FIXATION; PESTICIDES; PSEUDOMONAS; RHIZOBIUM; SOILS; CHEMICAL REACTIONS; DECOMPOSITION; GROWTH; 540220* - Environment, Terrestrial- Chemicals Monitoring & Transport- (1990-); 550700 - Microbiology

Citation Formats

Kingsley, M T, Metting, F B, Fredrickson, J K, and Seidler, R J. Environmental restoration using plant-microbe bioaugmentation. United States: N. p., 1993. Web.
Kingsley, M T, Metting, F B, Fredrickson, J K, & Seidler, R J. Environmental restoration using plant-microbe bioaugmentation. United States.
Kingsley, M T, Metting, F B, Fredrickson, J K, and Seidler, R J. 1993. "Environmental restoration using plant-microbe bioaugmentation". United States. https://www.osti.gov/servlets/purl/6591767.
@article{osti_6591767,
title = {Environmental restoration using plant-microbe bioaugmentation},
author = {Kingsley, M T and Metting, F B and Fredrickson, J K and Seidler, R J},
abstractNote = {Land farming, for the purpose of bioremediation, refers traditionally to the spreading of contaminated soil, sediments, or other material over land; mechanically mixing it; incorporating various amendments, such as fertilizer or mulch; and sometimes inoculating with degradative microorganisms. Populations of bacteria added to soils often decline rapidly and become metabolically inactive. To efficiently degrade contaminants, microorganisms must be metabolically active. Thus, a significant obstacle to the successful use of microorganisms for environmental applications is their long-term survival and the expression of their degradative genes in situ. Rhizosphere microorganisms are known to be more metabolically active than those in bulk soil, because they obtain carbon and energy from root exudates and decaying root matter. Rhizosphere populations are also more abundant, often containing 10[sup 8] or more culturable bacteria per gram of soil, and bacterial populations on the rhizoplane can exceed 10[sup 9]/g root. Many of the critical parameters that influence the competitive ability of rhizosphere bacteria have not been identified, but microorganisms have frequently been introduced into soil (bioaugmentation) as part of routine or novel agronomic practices. However, the use of rhizosphere bacteria and their in situ stimulation by plant roots for degrading organic contaminants has received little attention. Published studies have demonstrated the feasibility of using rhizobacteria (Pseudomonas putida) for the rapid removal of chlorinated pesticides from contaminated soil, and to promote germination of radish seeds in the presence of otherwise phytotoxic levels of the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D), and phenoxyacetic acid (PAA). The present investigation was undertaken to determine if these strains (Pseudomonas putida PP0301/pRO101 and PP0301/pRO103) could be used to bioremediate 2,4-D-amended soil via plant-microbe bioaugmentation.},
doi = {},
url = {https://www.osti.gov/biblio/6591767}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Thu Apr 01 00:00:00 EST 1993},
month = {Thu Apr 01 00:00:00 EST 1993}
}

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