In vitro radiation studies on Ewing's sarcoma cell lines and human bone marrow: application to the clinical use of total body irradiation (TBI)

Journal Article · Sun Jul 01 00:00:00 EDT 1984 · Int. J. Radiat. Oncol., Biol. Phys.; (United States)

DOI:https://doi.org/10.1016/0360-3016(84)90171-8· OSTI ID:6579365

Kinsella, T J; Mitchell, J B; McPherson, S; Miser, J; Triche, T; Glatstein, E

Patients with Ewing's sarcoma who present with a central axis or proximal extremity primary and/or with metastatic disease have a poor prognosis despite aggressive combination chemotherapy and local irradiation. In this high risk group of patients, total body irradiation (TBI) has been proposed as a systemic adjuvant. To aid in the design of a clinical TBI protocol, the authors have studied in the in vitro radiation response of two established cell lines of Ewing's sarcoma and human bone marrow CFUc. The Ewing's lines showed a larger D/sub 0/ and anti-n compared to the bone marrow CFU. No repair of potentially lethal radiation damage (PLDR) was found after 4.5 Gy in plateau phase Ewing's sarcoma cells. A theoretical split dose survival curve for both the Ewing's sarcoma lines and human bone marrow CFUc using this TBI schedule shows a significantly lower surviving fraction (10/sup -4/-10/sup -5/) for the bone marrow CFUc. Based on these in vitro results, two 4.0 Gy fractions separated by 24 hours is proposed as the TBI regimen. Because of the potentially irreversible damage to bone marrow, autologous bone marrow transplantation following the TBI is felt to be necessary. The details of this clinical protocol in high risk Ewing's sarcoma patients are outlined.

• Research Organization: National Cancer Inst., Bethesda, MD
• OSTI ID: 6579365
• Journal Information: Int. J. Radiat. Oncol., Biol. Phys.; (United States), Vol. 10:7
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
62 RADIOLOGY AND NUCLEAR MEDICINE
BONE MARROW CELLS
BIOLOGICAL RADIATION EFFECTS
CELL KILLING
RADIOINDUCTION
TUMOR CELLS
BIOLOGICAL REPAIR
CFU
FRACTIONATED IRRADIATION
IN VITRO
MEV RANGE 01-10
SARCOMAS
SURVIVAL CURVES
WHOLE-BODY IRRADIATION
X RADIATION
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
CONNECTIVE TISSUE CELLS
DISEASES
ELECTROMAGNETIC RADIATION
ENERGY RANGE
EXTERNAL IRRADIATION
IONIZING RADIATIONS
IRRADIATION
MEV RANGE
NEOPLASMS
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
SOMATIC CELLS
560121* - Radiation Effects on Cells- External Source- (-1987)
550603 - Medicine- External Radiation in Therapy- (1980-)