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Title: Catalytic center of lecithin:cholesterol acyltransferase: isolation and sequence of diisopropyl fluorophosphate-labeled peptides

Abstract

Lecithin:cholesterol acyltransferase (LCAT) was purified from hog plasma and subsequently reacted with (/sup 3/H)-Diisopropyl fluorophosphate (DFP). The labeled enzyme was digested with pepsin and the peptides separated by high performance liquid chromatography (HPLC). Two radioactive peptides were isolated, subjected to automated amino acid sequencing and yielded the following data: A) Ile-Ser-Leu-Gly-Ala-Pro-Trp-Gly-Gly-Ser, and B) Tyr-Ile-Phe-Asp-x-Gly-Phe-Pro-Tyr-x-Asp-Pro-Val. Both of these sequences represent very highly conserved regions of the enzyme when compared to the sequence of human LCAT. Peptide (A) is considered to represent the catalytic center of LCAT based on comparisons with data reported in the literature.

Authors:
; ; ;
Publication Date:
Research Org.:
North Texas State Univ., Forth Worth
OSTI Identifier:
6576972
Resource Type:
Journal Article
Journal Name:
Biochem. Biophys. Res. Commun.; (United States)
Additional Journal Information:
Journal Volume: 1
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; PEPTIDES; AMINO ACID SEQUENCE; TRANSFERASES; FRACTIONATION; LIQUID COLUMN CHROMATOGRAPHY; SWINE; TRACER TECHNIQUES; TRITIUM COMPOUNDS; ANIMALS; CHROMATOGRAPHY; DOMESTIC ANIMALS; ENZYMES; ISOTOPE APPLICATIONS; LABELLED COMPOUNDS; MAMMALS; MOLECULAR STRUCTURE; ORGANIC COMPOUNDS; PROTEINS; SEPARATION PROCESSES; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Park, Y B, Yueksel, U G, Gracy, R W, and Lacko, A G. Catalytic center of lecithin:cholesterol acyltransferase: isolation and sequence of diisopropyl fluorophosphate-labeled peptides. United States: N. p., 1987. Web. doi:10.1016/0006-291X(87)90673-5.
Park, Y B, Yueksel, U G, Gracy, R W, & Lacko, A G. Catalytic center of lecithin:cholesterol acyltransferase: isolation and sequence of diisopropyl fluorophosphate-labeled peptides. United States. https://doi.org/10.1016/0006-291X(87)90673-5
Park, Y B, Yueksel, U G, Gracy, R W, and Lacko, A G. 1987. "Catalytic center of lecithin:cholesterol acyltransferase: isolation and sequence of diisopropyl fluorophosphate-labeled peptides". United States. https://doi.org/10.1016/0006-291X(87)90673-5.
@article{osti_6576972,
title = {Catalytic center of lecithin:cholesterol acyltransferase: isolation and sequence of diisopropyl fluorophosphate-labeled peptides},
author = {Park, Y B and Yueksel, U G and Gracy, R W and Lacko, A G},
abstractNote = {Lecithin:cholesterol acyltransferase (LCAT) was purified from hog plasma and subsequently reacted with (/sup 3/H)-Diisopropyl fluorophosphate (DFP). The labeled enzyme was digested with pepsin and the peptides separated by high performance liquid chromatography (HPLC). Two radioactive peptides were isolated, subjected to automated amino acid sequencing and yielded the following data: A) Ile-Ser-Leu-Gly-Ala-Pro-Trp-Gly-Gly-Ser, and B) Tyr-Ile-Phe-Asp-x-Gly-Phe-Pro-Tyr-x-Asp-Pro-Val. Both of these sequences represent very highly conserved regions of the enzyme when compared to the sequence of human LCAT. Peptide (A) is considered to represent the catalytic center of LCAT based on comparisons with data reported in the literature.},
doi = {10.1016/0006-291X(87)90673-5},
url = {https://www.osti.gov/biblio/6576972}, journal = {Biochem. Biophys. Res. Commun.; (United States)},
number = ,
volume = 1,
place = {United States},
year = {Fri Feb 27 00:00:00 EST 1987},
month = {Fri Feb 27 00:00:00 EST 1987}
}