Neurogenesis in the vomeronasal epithelium of adult garter snakes: 3. Use of /sup 3/H-thymidine autoradiography to trace the genesis and migration of bipolar neurons
Use of 3H-thymidine autoradiography and unilateral vomeronasal (VN) axotomy has permitted us to demonstrate directly the existence of VN stem cells in the adult garter snake and to trace continuous bipolar neuron development and migration in the normal VN and deafferentated VN epithelium in the same animal. The vomeronasal epithelium and olfactory epithelium of adult garter snakes are both capable of incorporating 3H-thymidine. In the sensory epithelium of the vomeronasal organ, 3H-thymidine-labeled cells were initially restricted to the base of the undifferentiated cell layer in animals surviving 1 day following 3H-thymidine injection. With increasing survival time, labeled cells progressively migrated vertically within the receptor cell column toward the apex of the bipolar neuron layer. In both the normal and denervated VN epithelium, labeled cells were observed through the 56 days of postoperative survival. In the normal epithelium, labeled cells were always located within the matrix of the intact receptor cell columns. However, labeled cells of the denervated epithelium were always located at the apical front of the newly formed cell mass following depletion of the original neuronal cell population. In addition, at postoperative days 28 and 56, labeled cells of the denervated VN epithelium achieved neuronal differentiation and maturation by migrating much farther away from the base of the receptor cell column than the labeled cells on the normal, unoperated contralateral side. This study directly demonstrates that basal cells initially incorporating 3H-thymidine are indeed stem cells of the VN epithelium in adult garter snakes.
- Research Organization:
- Marshall Univ. School of Medicine, Huntington, WV (USA)
- OSTI ID:
- 6569265
- Journal Information:
- Am. J. Anat.; (United States), Vol. 183:2
- Country of Publication:
- United States
- Language:
- English
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NERVE CELLS
CELL DIFFERENTIATION
AUTORADIOGRAPHY
CHEMORECEPTORS
EPITHELIUM
OLFACTORY BULBS
SNAKES
STEM CELLS
SURVIVAL TIME
THYMIDINE
TRITIUM COMPOUNDS
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
AZINES
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
HETEROCYCLIC COMPOUNDS
LABELLED COMPOUNDS
NERVOUS SYSTEM
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PYRIMIDINES
REPTILES
RIBOSIDES
SOMATIC CELLS
TISSUES
VERTEBRATES
550301* - Cytology- Tracer Techniques