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Title: In vivo distribution and excretion studies and in vitro blood studies on the kinetics of lead-203 in beagle dogs

Abstract

The distribution of carrier free lead-203 between plasma and cells of canine blood in vitro was measured. Activity in the plasma decreased to less than 5% of the initial blood activity during the first 15 min and then exponentially with a half time of 160 min. Incubation in cell free plasma before addition to whole blood transformed the isotope, decreasing the amount subsequently associated with cells. In animal studies, activity was measured in plasma, blood cells, urine, and feces after exposure to lead-203. In one group, the animals were exposed by intravenous injection of dilute citric acid solutions of isotope. In a second group, carrier free isotope which had been transformed by incubation in plasma was injected intravenously. The kinetics of the distribution of the isotope differed between the two experimental groups. After injection of the transformed lead, the lead-203 content of the blood cell fraction rose from 10% of the dose at 5 min to 21% to 43% at 13 h. The red cell activity after injection of citric acid solutions of lead-203 remained between 50 and 60% of the dose from 5 min to 12 h post exposure. Excretion of lead in the urine during the first daymore » after injection of transformed lead ranged from 5 to 45% of the dose, while that of citric acid solutions of lead was between 1 and 2%. Linear compartmental models for distribution of the isotope were developed for both in vitro and in vivo experimental systems. In three additional experiments, animals were exposed to lead-203 extravascularly. In one, using a dilute citric acid solution of the isotope, plasma activity resembled that observed after intravenous injection, suggesting that isotope was transformed by extravascular fluids before reaching the circulation. In the two remaining experiments the animals were exposed to lead-203 by gavage. The results suggest that lead absorbed from the gut may have been transformed before reaching the circulation. (ERB)« less

Authors:
 [1]
  1. Rochester Univ., NY (United States). Dept. of Radiation Biology and Biophysics
Publication Date:
Research Org.:
Rochester Univ., NY (United States). Dept. of Radiation Biology and Biophysics
Sponsoring Org.:
USDOE; US Energy Research and Development Administration (ERDA)
OSTI Identifier:
6564204
Report Number(s):
UR-3490-1883
DOE Contract Number:  
AC02-76EV03490
Resource Type:
Thesis/Dissertation
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 59 BASIC BIOLOGICAL SCIENCES; BEAGLES; RADIONUCLIDE KINETICS; BLOOD PLASMA; ERYTHROCYTES; LEAD 203; EXCRETION; TISSUE DISTRIBUTION; CARRIER-FREE ISOTOPES; COMPARATIVE EVALUATIONS; IN VITRO; IN VIVO; INCUBATION; INTRAVENOUS INJECTION; MATHEMATICAL MODELS; ORAL ADMINISTRATION; SUBCUTANEOUS INJECTION; TRACER TECHNIQUES; ANIMALS; BETA DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CLEARANCE; DAYS LIVING RADIOISOTOPES; DISTRIBUTION; DOGS; ELECTRON CAPTURE RADIOISOTOPES; EVEN-ODD NUCLEI; HEAVY NUCLEI; INJECTION; INTAKE; ISOMERIC TRANSITION ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; LEAD ISOTOPES; MAMMALS; MATERIALS; NUCLEI; RADIOISOTOPES; SECONDS LIVING RADIOISOTOPES; VERTEBRATES; 560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987); 551001 - Physiological Systems- Tracer Techniques

Citation Formats

Coombs, Mark A. In vivo distribution and excretion studies and in vitro blood studies on the kinetics of lead-203 in beagle dogs. United States: N. p., 1980. Web. doi:10.2172/6564204.
Coombs, Mark A. In vivo distribution and excretion studies and in vitro blood studies on the kinetics of lead-203 in beagle dogs. United States. https://doi.org/10.2172/6564204
Coombs, Mark A. 1980. "In vivo distribution and excretion studies and in vitro blood studies on the kinetics of lead-203 in beagle dogs". United States. https://doi.org/10.2172/6564204. https://www.osti.gov/servlets/purl/6564204.
@article{osti_6564204,
title = {In vivo distribution and excretion studies and in vitro blood studies on the kinetics of lead-203 in beagle dogs},
author = {Coombs, Mark A.},
abstractNote = {The distribution of carrier free lead-203 between plasma and cells of canine blood in vitro was measured. Activity in the plasma decreased to less than 5% of the initial blood activity during the first 15 min and then exponentially with a half time of 160 min. Incubation in cell free plasma before addition to whole blood transformed the isotope, decreasing the amount subsequently associated with cells. In animal studies, activity was measured in plasma, blood cells, urine, and feces after exposure to lead-203. In one group, the animals were exposed by intravenous injection of dilute citric acid solutions of isotope. In a second group, carrier free isotope which had been transformed by incubation in plasma was injected intravenously. The kinetics of the distribution of the isotope differed between the two experimental groups. After injection of the transformed lead, the lead-203 content of the blood cell fraction rose from 10% of the dose at 5 min to 21% to 43% at 13 h. The red cell activity after injection of citric acid solutions of lead-203 remained between 50 and 60% of the dose from 5 min to 12 h post exposure. Excretion of lead in the urine during the first day after injection of transformed lead ranged from 5 to 45% of the dose, while that of citric acid solutions of lead was between 1 and 2%. Linear compartmental models for distribution of the isotope were developed for both in vitro and in vivo experimental systems. In three additional experiments, animals were exposed to lead-203 extravascularly. In one, using a dilute citric acid solution of the isotope, plasma activity resembled that observed after intravenous injection, suggesting that isotope was transformed by extravascular fluids before reaching the circulation. In the two remaining experiments the animals were exposed to lead-203 by gavage. The results suggest that lead absorbed from the gut may have been transformed before reaching the circulation. (ERB)},
doi = {10.2172/6564204},
url = {https://www.osti.gov/biblio/6564204}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jan 01 00:00:00 EST 1980},
month = {Tue Jan 01 00:00:00 EST 1980}
}

Thesis/Dissertation:
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