Direct antimicrobial drug susceptibility testing of Mycobacterium tuberculosis by the radiometric method
Direct-drug-susceptibility tests were performed on clinical specimens positive for acid-fast bacilli by either Ziehl-Neelsen or fluorochrome staining. The results of conventional agar dilution and a modified radiometric (BACTEC) method were compared. A total of 580 smear-positive specimens were tested by the BACTEC method at three separate sites. Three hundred and seventy-seven of these were culture positive for M. tuberculosis, and 343 (91%) yielded acceptable direct-susceptibility-test results. We used the conventional method to determine that 343 of 519 smear-positive specimens were culture positive for M. tuberculosis, and 212 (62%) produced acceptable results within 3 wks. Conventional results were reported in 3-4 wks, while the time required to obtain results with the BACTEC method ranged from 5 to 21 days (average 11.5 days). Results indicate that the radiometric method provides reportable results more frequently with time savings as compared to the conventional method.
- Research Organization:
- Maryland Department of Health and Mental Hygiene, Baltimore (USA)
- OSTI ID:
- 6560367
- Journal Information:
- Diagn. Microbiol. Infect. Dis.; (United States), Vol. 10:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
BACTERIAL DISEASES
DIAGNOSIS
MYCOBACTERIUM TUBERCULOSIS
RADIOMETRIC ANALYSIS
ISONIAZID
STREPTOMYCIN
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIMICROBIAL AGENTS
BACTERIA
CHEMICAL ANALYSIS
DISEASES
DRUGS
HYDRAZIDES
INFECTIOUS DISEASES
MICROORGANISMS
MYCOBACTERIUM
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
QUANTITATIVE CHEMICAL ANALYSIS
550601* - Medicine- Unsealed Radionuclides in Diagnostics
550901 - Pathology- Tracer Techniques