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Title: Characterization of antigens from Schistosoma mansoni and construction of a cDNA library for the study of schistosomiasis

Abstract

To examine the antigens of adult Schistosoma mansoni, /sup 35/S-methionine-labelled, detergent-extracted proteins were immunoprecipitated and analyzed on SDS-PAGE. Human infection serum immunoprecipitated 14 polypeptides with M/sub r/'s of 120, 105, 88, 86, 66, 64, 54, 48, 42, 38, 35, 32, 29, and 20 Kd. Upon digestion with endoglycosidase F polypeptides with M/sub r/'s of 120, 105, 54, 48, and 29 appeared to have carbohydrate moieties. Extracts of female and male S. mansoni were analyzed by immunoprecipitation and immunoblotting. Two polypeptides with M/sub r/'s of 86 Kd and 54 Kd were detected only in extracts of males. Polyadenylated RNA was extracted from S. mansoni and translated in rabbit reticulocyte lysates. Among the in vitro translation products, polypeptides with 120, 94, 64, 43, 37, 35, 30, 26 and 22 Kd apparent molecular weights were immunoprecipitated by human infection serum. When the translation products of female worms and male worms were compared, the polypeptides with M/sub r/'s of 94 and 64 Kd were only observed in males.

Authors:
Publication Date:
Research Org.:
California Univ., Los Angeles (USA)
OSTI Identifier:
6550460
Resource Type:
Thesis/Dissertation
Resource Relation:
Other Information: Thesis (Ph. D)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ANTIGENS; CHEMICAL COMPOSITION; POLYPEPTIDES; ELECTROPHORESIS; SCHISTOSOMA; SCHISTOSOMIASIS; DNA-CLONING; PATHOGENESIS; DNA; IMMUNE REACTIONS; METHIONINE; MOLECULAR WEIGHT; RNA; SEX DEPENDENCE; SULFUR 35; TRACER TECHNIQUES; AMINO ACIDS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CARBOXYLIC ACIDS; CLONING; DAYS LIVING RADIOISOTOPES; DISEASES; DRUGS; EVEN-ODD NUCLEI; HELMINTHS; INFECTIOUS DISEASES; ISOTOPE APPLICATIONS; ISOTOPES; LIGHT NUCLEI; LIPOTROPIC FACTORS; NUCLEI; NUCLEIC ACIDS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC SULFUR COMPOUNDS; PARASITIC DISEASES; PEPTIDES; PLATYHELMINTHS; PROTEINS; RADIOISOTOPES; SULFUR ISOTOPES; TREMATODES; 550201* - Biochemistry- Tracer Techniques; 550901 - Pathology- Tracer Techniques

Citation Formats

Bugra, K. Characterization of antigens from Schistosoma mansoni and construction of a cDNA library for the study of schistosomiasis. United States: N. p., 1986. Web.
Bugra, K. Characterization of antigens from Schistosoma mansoni and construction of a cDNA library for the study of schistosomiasis. United States.
Bugra, K. 1986. "Characterization of antigens from Schistosoma mansoni and construction of a cDNA library for the study of schistosomiasis". United States.
@article{osti_6550460,
title = {Characterization of antigens from Schistosoma mansoni and construction of a cDNA library for the study of schistosomiasis},
author = {Bugra, K},
abstractNote = {To examine the antigens of adult Schistosoma mansoni, /sup 35/S-methionine-labelled, detergent-extracted proteins were immunoprecipitated and analyzed on SDS-PAGE. Human infection serum immunoprecipitated 14 polypeptides with M/sub r/'s of 120, 105, 88, 86, 66, 64, 54, 48, 42, 38, 35, 32, 29, and 20 Kd. Upon digestion with endoglycosidase F polypeptides with M/sub r/'s of 120, 105, 54, 48, and 29 appeared to have carbohydrate moieties. Extracts of female and male S. mansoni were analyzed by immunoprecipitation and immunoblotting. Two polypeptides with M/sub r/'s of 86 Kd and 54 Kd were detected only in extracts of males. Polyadenylated RNA was extracted from S. mansoni and translated in rabbit reticulocyte lysates. Among the in vitro translation products, polypeptides with 120, 94, 64, 43, 37, 35, 30, 26 and 22 Kd apparent molecular weights were immunoprecipitated by human infection serum. When the translation products of female worms and male worms were compared, the polypeptides with M/sub r/'s of 94 and 64 Kd were only observed in males.},
doi = {},
url = {https://www.osti.gov/biblio/6550460}, journal = {},
number = ,
volume = ,
place = {United States},
year = {1986},
month = {1}
}

Thesis/Dissertation:
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