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Title: Alterations of 86Rb+ fluxes in poliovirus-infected HeLa cells and their dependence on virus replication

Abstract

Components of the 86Rb+ influx were investigated subsequent to poliovirus infection in the presence and absence of guanidine-HCl, both under normal steady-state conditions and after Na+ preloading of the cells. Measurements of the ouabain-sensitive 86Rb+ uptake indicated a biphasic change in the activity of the Na+, K+ pump in the course of virus infection: a transient increase in the second hour postinfection, that was detectable only after Na+ preloading and inhibition after 3 hr. The enhanced activity of the Na+, K+ pump was not affected, while the decrease later was fully prevented by the antiviral agent guanidine-HCl. The piretanide-sensitive 86Rb+ uptake due to the Na+, K+, 2 Cl- cotransport system also became strongly inhibited beginning in the second hour postinfection. The inhibition of this transport system was partially antagonized by guanidine-HCl. The remaining 86Rb+ influx in the presence of ouabain and piretanide increased in the third hour postinfection. The latter change in 86Rb+ influx, indicating an increased permeability to monovalent cations was completely abolished by guanidine-HCl.

Authors:
; ; ; ;
Publication Date:
Research Org.:
Abteilung Molekularbiologie, Physiologisch-Chemisches Institut, Universitaet Hamburg, Federal Republic of Germany
OSTI Identifier:
6549804
Resource Type:
Journal Article
Journal Name:
Virology; (United States)
Additional Journal Information:
Journal Volume: 136:2
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; GUANIDINES; BIOLOGICAL EFFECTS; POLIO VIRUS; DNA REPLICATION; RUBIDIUM 86; UPTAKE; ATP-ASE; CATIONS; CELL MEMBRANES; HELA CELLS; MEMBRANE TRANSPORT; POTASSIUM COMPOUNDS; SODIUM COMPOUNDS; ACID ANHYDRASES; ALKALI METAL COMPOUNDS; ALKALI METAL ISOTOPES; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CARBONIC ACID DERIVATIVES; CELL CONSTITUENTS; CHARGED PARTICLES; DAYS LIVING RADIOISOTOPES; ENZYMES; HYDROLASES; INTERMEDIATE MASS NUCLEI; IONS; ISOMERIC TRANSITION ISOTOPES; ISOTOPES; MEMBRANES; MICROORGANISMS; MINUTES LIVING RADIOISOTOPES; NUCLEI; NUCLEIC ACID REPLICATION; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PARASITES; PHOSPHOHYDROLASES; RADIOISOTOPES; RUBIDIUM ISOTOPES; VIRUSES; 550701* - Microbiology- Tracer Techniques

Citation Formats

Schaefer, A., Geck, P., Zibirre, R., Kuehne, J., and Koch, G. Alterations of 86Rb+ fluxes in poliovirus-infected HeLa cells and their dependence on virus replication. United States: N. p., 1984. Web. doi:10.1016/0042-6822(84)90184-3.
Schaefer, A., Geck, P., Zibirre, R., Kuehne, J., & Koch, G. Alterations of 86Rb+ fluxes in poliovirus-infected HeLa cells and their dependence on virus replication. United States. doi:10.1016/0042-6822(84)90184-3.
Schaefer, A., Geck, P., Zibirre, R., Kuehne, J., and Koch, G. Mon . "Alterations of 86Rb+ fluxes in poliovirus-infected HeLa cells and their dependence on virus replication". United States. doi:10.1016/0042-6822(84)90184-3.
@article{osti_6549804,
title = {Alterations of 86Rb+ fluxes in poliovirus-infected HeLa cells and their dependence on virus replication},
author = {Schaefer, A. and Geck, P. and Zibirre, R. and Kuehne, J. and Koch, G.},
abstractNote = {Components of the 86Rb+ influx were investigated subsequent to poliovirus infection in the presence and absence of guanidine-HCl, both under normal steady-state conditions and after Na+ preloading of the cells. Measurements of the ouabain-sensitive 86Rb+ uptake indicated a biphasic change in the activity of the Na+, K+ pump in the course of virus infection: a transient increase in the second hour postinfection, that was detectable only after Na+ preloading and inhibition after 3 hr. The enhanced activity of the Na+, K+ pump was not affected, while the decrease later was fully prevented by the antiviral agent guanidine-HCl. The piretanide-sensitive 86Rb+ uptake due to the Na+, K+, 2 Cl- cotransport system also became strongly inhibited beginning in the second hour postinfection. The inhibition of this transport system was partially antagonized by guanidine-HCl. The remaining 86Rb+ influx in the presence of ouabain and piretanide increased in the third hour postinfection. The latter change in 86Rb+ influx, indicating an increased permeability to monovalent cations was completely abolished by guanidine-HCl.},
doi = {10.1016/0042-6822(84)90184-3},
journal = {Virology; (United States)},
number = ,
volume = 136:2,
place = {United States},
year = {1984},
month = {7}
}