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Title: Bioengineering of photosynthetic membranes. Requirement of magnesium for the conversion of chlorophyllide a to chlorophyll a during the greening of etiochloroplasts in vitro

Abstract

The massive conversion of delta-aminolevulinic acid (ALA) to protochlorophyllide (Pchlide) and the massive conversion of chlorophyllide a (Chlide a) to chlorophyll a (Chl a) are two essential conditions for the ALA-dependent assembly of photosynthetic membranes in vitro. In this work, the authors describe the development of a cell-free system capable of the forementioned biosynthetic activities at rates higher than in vivo, for the first 2 h of dark-incubation. The cell-free system consisted of 1) etiochloroplasts prepared from kinetin and gibberellic-acid-pretreated cucumber cotyledons, and 2) cofactors and additives described elsewhere and which are needed for the massive conversion of ALA to Pchlide, 3) high concentrations of ATP, MgCl/sub 2/, and an isoprenol alcohol such as phytol, were required for the massive conversion of Chlide a to Chl a. An absolute and novel requirement of Mg/sup 2 +/ for the conversion of Chlide a to Chl a was also demonstrated. In addition to the role of phytol as a substrate for the conversion of Chlide a to Chl a, the data suggested that this alcohol may also be involved in the regulation of the reactions between ALA and Pchlide. It is proposed that during greening, the conversion of Chlide a to Chlmore » a may follow different biosynthetic rates, having different substrate and cofactor requirements, depending on the stage of plastid development.« less

Authors:
;
Publication Date:
Research Org.:
Univ. of Illinois, Urbana
OSTI Identifier:
6546973
Resource Type:
Journal Article
Journal Name:
Biotechnol. Bioeng.; (United States)
Additional Journal Information:
Journal Volume: 26:5
Country of Publication:
United States
Language:
English
Subject:
14 SOLAR ENERGY; CHLOROPHYLL; BIOCONVERSION; BIOMIMETIC PROCESSES; MAGNESIUM; USES; PHOTOSYNTHETIC MEMBRANES; CHEMICAL PREPARATION; AMINOLEVULINIC ACID; CUCUMBERS; IN VITRO; KINETIN; SEEDS; ADENINES; ALKALINE EARTH METALS; AMINES; AMINO ACIDS; ANTIMETABOLITES; AROMATICS; AZAARENES; CARBOXYLIC ACIDS; DRUGS; ELEMENTS; FOOD; HETEROCYCLIC ACIDS; HETEROCYCLIC COMPOUNDS; MEMBRANES; METALS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PHYTOCHROMES; PIGMENTS; PLANTS; PORPHYRINS; PURINES; SYNTHESIS; VEGETABLES; 140505* - Solar Energy Conversion- Photochemical, Photobiological, & Thermochemical Conversion- (1980-)

Citation Formats

Daniell, H, and Rebeiz, C A. Bioengineering of photosynthetic membranes. Requirement of magnesium for the conversion of chlorophyllide a to chlorophyll a during the greening of etiochloroplasts in vitro. United States: N. p., 1984. Web. doi:10.1002/bit.260260512.
Daniell, H, & Rebeiz, C A. Bioengineering of photosynthetic membranes. Requirement of magnesium for the conversion of chlorophyllide a to chlorophyll a during the greening of etiochloroplasts in vitro. United States. https://doi.org/10.1002/bit.260260512
Daniell, H, and Rebeiz, C A. 1984. "Bioengineering of photosynthetic membranes. Requirement of magnesium for the conversion of chlorophyllide a to chlorophyll a during the greening of etiochloroplasts in vitro". United States. https://doi.org/10.1002/bit.260260512.
@article{osti_6546973,
title = {Bioengineering of photosynthetic membranes. Requirement of magnesium for the conversion of chlorophyllide a to chlorophyll a during the greening of etiochloroplasts in vitro},
author = {Daniell, H and Rebeiz, C A},
abstractNote = {The massive conversion of delta-aminolevulinic acid (ALA) to protochlorophyllide (Pchlide) and the massive conversion of chlorophyllide a (Chlide a) to chlorophyll a (Chl a) are two essential conditions for the ALA-dependent assembly of photosynthetic membranes in vitro. In this work, the authors describe the development of a cell-free system capable of the forementioned biosynthetic activities at rates higher than in vivo, for the first 2 h of dark-incubation. The cell-free system consisted of 1) etiochloroplasts prepared from kinetin and gibberellic-acid-pretreated cucumber cotyledons, and 2) cofactors and additives described elsewhere and which are needed for the massive conversion of ALA to Pchlide, 3) high concentrations of ATP, MgCl/sub 2/, and an isoprenol alcohol such as phytol, were required for the massive conversion of Chlide a to Chl a. An absolute and novel requirement of Mg/sup 2 +/ for the conversion of Chlide a to Chl a was also demonstrated. In addition to the role of phytol as a substrate for the conversion of Chlide a to Chl a, the data suggested that this alcohol may also be involved in the regulation of the reactions between ALA and Pchlide. It is proposed that during greening, the conversion of Chlide a to Chl a may follow different biosynthetic rates, having different substrate and cofactor requirements, depending on the stage of plastid development.},
doi = {10.1002/bit.260260512},
url = {https://www.osti.gov/biblio/6546973}, journal = {Biotechnol. Bioeng.; (United States)},
number = ,
volume = 26:5,
place = {United States},
year = {1984},
month = {1}
}