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Title: Microbial solubilization of coals

Abstract

Microbial solubilization of coal may serve as a first step in a process to convert low-rank coals or coal-derived products to other fuels or products. For solubilization of coal to be an economically viable technology, a mechanistic understanding of the process is essential. Leonardite, a highly oxidized, low-rank coal, has been solubilized by the intact microorganism, cell-free filtrate, and cell-free enzyme of /ital Coriolus versicolor/. A spectrophotometric conversion assay was developed to quantify the amount of biosolubilized coal. In addition, a bituminous coal, Illinois No. 6, was solubilized by a species of /ital Penicillium/, but only after the coal had been preoxidized in air. Model compounds containing coal-related functionalities have been incubated with the leonardite-degrading fungus, its cell-free filtrate, and purified enzyme. The amount of degradation was determined by gas chromatography and the degradation products were identified by gas chromatography/mass spectrometry. We have also separated the cell-free filtrate of /ital C. versicolor/ into a <10,000 MW and >10,000 MW fraction by ultrafiltration techniques. Most of the coal biosolubilization activity is contained in the <10,000 MW fraction while the model compound degradation occurs in the >10,000 MW fraction. The >10,000 MW fraction appears to contain an enzyme with laccase-like activity. 10more » refs., 8 figs., 5 tabs.« less

Authors:
; ; ; ; ; ;
Publication Date:
Research Org.:
Pacific Northwest Lab., Richland, WA (USA)
OSTI Identifier:
6546911
Report Number(s):
PNL-SA-16459; CONF-881205-1
ON: DE89005160
DOE Contract Number:  
AC06-76RL01830
Resource Type:
Conference
Resource Relation:
Conference: Gas, oil and coal biotechnology symposium, New Orleans, LA, USA, 5 Dec 1988; Other Information: Portions of this document are illegible in microfiche products
Country of Publication:
United States
Language:
English
Subject:
01 COAL, LIGNITE, AND PEAT; 59 BASIC BIOLOGICAL SCIENCES; COAL; BIODEGRADATION; CARBON 13; GEL PERMEATION CHROMATOGRAPHY; MICROORGANISMS; MOLECULAR WEIGHT; NUCLEAR MAGNETIC RESONANCE; SOLUBILITY; CARBON ISOTOPES; CARBONACEOUS MATERIALS; CHEMICAL REACTIONS; CHROMATOGRAPHY; DECOMPOSITION; ENERGY SOURCES; EVEN-ODD NUCLEI; FOSSIL FUELS; FUELS; ISOTOPES; LIGHT NUCLEI; MAGNETIC RESONANCE; MATERIALS; NUCLEI; RESONANCE; SEPARATION PROCESSES; STABLE ISOTOPES; 010400* - Coal, Lignite, & Peat- Processing; 550700 - Microbiology

Citation Formats

Campbell, J A, Fredrickson, J K, Stewart, D L, Thomas, B L, McCulloch, M, Wilson, B W, and Bean, R M. Microbial solubilization of coals. United States: N. p., 1988. Web.
Campbell, J A, Fredrickson, J K, Stewart, D L, Thomas, B L, McCulloch, M, Wilson, B W, & Bean, R M. Microbial solubilization of coals. United States.
Campbell, J A, Fredrickson, J K, Stewart, D L, Thomas, B L, McCulloch, M, Wilson, B W, and Bean, R M. 1988. "Microbial solubilization of coals". United States.
@article{osti_6546911,
title = {Microbial solubilization of coals},
author = {Campbell, J A and Fredrickson, J K and Stewart, D L and Thomas, B L and McCulloch, M and Wilson, B W and Bean, R M},
abstractNote = {Microbial solubilization of coal may serve as a first step in a process to convert low-rank coals or coal-derived products to other fuels or products. For solubilization of coal to be an economically viable technology, a mechanistic understanding of the process is essential. Leonardite, a highly oxidized, low-rank coal, has been solubilized by the intact microorganism, cell-free filtrate, and cell-free enzyme of /ital Coriolus versicolor/. A spectrophotometric conversion assay was developed to quantify the amount of biosolubilized coal. In addition, a bituminous coal, Illinois No. 6, was solubilized by a species of /ital Penicillium/, but only after the coal had been preoxidized in air. Model compounds containing coal-related functionalities have been incubated with the leonardite-degrading fungus, its cell-free filtrate, and purified enzyme. The amount of degradation was determined by gas chromatography and the degradation products were identified by gas chromatography/mass spectrometry. We have also separated the cell-free filtrate of /ital C. versicolor/ into a <10,000 MW and >10,000 MW fraction by ultrafiltration techniques. Most of the coal biosolubilization activity is contained in the <10,000 MW fraction while the model compound degradation occurs in the >10,000 MW fraction. The >10,000 MW fraction appears to contain an enzyme with laccase-like activity. 10 refs., 8 figs., 5 tabs.},
doi = {},
url = {https://www.osti.gov/biblio/6546911}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Nov 01 00:00:00 EST 1988},
month = {Tue Nov 01 00:00:00 EST 1988}
}

Conference:
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