Identification and comparative genotoxicity of polycyclic aromatic hydrocarbons and related nitrogen-containing heteroatomic species in products from coal liquefaction processes

Technical Report · Tue Feb 01 00:00:00 EST 1983

OSTI ID:6546696

Later, D W; Pelroy, R A; Mahlum, D D; Wright, C W; Lee, M L; Wilson, B W

A series of process distillation cuts from a solvent refined coal process were separated into polycyclic aromatic hydrocarbons (PAH) and nitrogen-containing polycyclic aromatic hydrocarbons (N-PAC) chemical classes and the fractions tested for biological activity by both microbial mutagenicity and mammalian skin carcinogenicity assays. Qualitative and quantitative chemical characterization of individual PAH and N-PAC in these materials was also performed. The results of biological and chemical analyses are presented in this paper and used to assess the comparative mutagenicity of the PAH and N-PAC from complex mixtures of coal liquefaction materials. Chemical class separation was obtained using adsorption column chromatography. Gas chromatography and mass spectroscopy were used to identify the fractions. The following PAH compounds were identified and quantitiated: phenanthrene, fluoranthene, pyrene, benzoanthracene, chrysene, methylchrysens, benzo(j) or (b)fluoranthene, benzo (k) fluoranthene, 7,12-Dimethyl-benzanthracene, benzopyrene, and benzoperylene. N-PAC compounds identified were: carbazole, benzo(a)carbazole, phenanthridine, 1-azapyrene, 2-azafluoranthene, benz(c)acridine, 2-aminofluorene, 3-aminophenanthrene, 2-aminoanthracene, 2-aminopyrene, 8-aminofluoranthene, 3-aminochrysene, and 7-aminobenzo(a)pyrene. In terms of microbial mutagenicity using the Ames test, the N-PAC, in general, are more active than the PAH; the principal mutagenic N-PAC agents being the amino-PAH. If carcinogenicity is investigated by the mouse skin initiation/promotion approach, the PAH are the most potent carcinogens with activity increasing with increasing distillation temperature. (DMC)

• Research Organization: Pacific Northwest Lab., Richland, WA (USA)
• DOE Contract Number: AC06-76RL01830
• OSTI ID: 6546696
• Report Number(s): PNL-SA-10869; CONF-8210100-2; ON: DE83006939
• Resource Relation: Conference: 7. international symposium on polynuclear aromatic hydrocarbons, Columbus, OH, USA, 26 Oct 1982
• Country of Publication: United States
• Language: English

Related Subjects

01 COAL, LIGNITE, AND PEAT
37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
COAL LIQUIDS
FRACTIONATION
ORGANIC NITROGEN COMPOUNDS
QUALITATIVE CHEMICAL ANALYSIS
TOXICITY
POLYCYCLIC AROMATIC HYDROCARBONS
CARCINOGEN SCREENING
CHEMICAL ANALYSIS
GENETIC EFFECTS
MAMMALS
MUTAGEN SCREENING
QUANTITATIVE CHEMICAL ANALYSIS
SALMONELLA TYPHIMURIUM
SKIN
ANIMALS
AROMATICS
BACTERIA
BIOLOGICAL EFFECTS
BODY
FLUIDS
HYDROCARBONS
LIQUIDS
MICROORGANISMS
ORGANIC COMPOUNDS
ORGANS
SALMONELLA
SCREENING
SEPARATION PROCESSES
VERTEBRATES
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