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Title: Serum protein inhibition of thyrotropin binding to human thyroid tissue. [/sup 125/I tracer technique]

Abstract

We used a modificaton of the TSH radioreceptor assay to detect TSH-binding inhibition (TBI) activity in serum and serum fractions from normal subjects and patients with Graves' disease. TBI activity is present in normal IgG prepared by DEAE-Sephadex chromatography and in normal globulins prepared by precipitation at 1.6 M ammonium sulfate. Other normal serum proteins also had TBI activity when large concentrations were tested. Gel filtration chromatography and powder block electrophoresis were used to prepare fractions of normal and Graves' disease sera. In these fractions from normal serum, TBI activity was found in both ..gamma..-globulin and ..cap alpha..-globulin-albumin fractions electrophoretically and in both 7S and 4S peaks from gel filtration. TBI activity from Graves' disease patients' sera was similarly distributed, but relatively more TBI accompanied the electrophoretic ..gamma..-globulins. Sepharose Protein-A and anti-IgG were used as immunoabsorbents to isolate and purify IgG from normal and Graves' disease sera. TBI activity in IgG was proportional to the IgG concentration, indicating that the TBI which migrates as a ..gamma..-globulin electrophoretically is an IgG and thus may possibly be an antibody. Inhibitory activity found in normal serum globulins and in the non-IgG fractions of both normal and abnormal sera seriously interferes with attempts tomore » use the TSH radioreceptor assay to study the hypothesized anti-TSH receptor antibody in the serum of patients with Graves' disease.« less

Authors:
; ; ;
Publication Date:
Research Org.:
Harbor General Hospital, Torrance, CA
OSTI Identifier:
6542704
Resource Type:
Journal Article
Journal Name:
J. Clin. Endocrinol. Metab.; (United States)
Additional Journal Information:
Journal Volume: 47:5
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; PROTEINS; BINDING ENERGY; THYROID; TSH; RADIOIMMUNOASSAY; BLOOD SERUM; IMMUNOGLOBULINS; INHIBITION; IODINE 125; PURIFICATION; TRACER TECHNIQUES; BETA DECAY RADIOISOTOPES; BODY; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; ENDOCRINE GLANDS; ENERGY; GLANDS; GLOBULINS; HORMONES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANS; PEPTIDE HORMONES; PITUITARY HORMONES; RADIOASSAY; RADIOISOTOPES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics

Citation Formats

Beall, G N, Chopra, I J, Solomon, D H, and Kruger, S R. Serum protein inhibition of thyrotropin binding to human thyroid tissue. [/sup 125/I tracer technique]. United States: N. p., 1978. Web. doi:10.1210/jcem-47-5-967.
Beall, G N, Chopra, I J, Solomon, D H, & Kruger, S R. Serum protein inhibition of thyrotropin binding to human thyroid tissue. [/sup 125/I tracer technique]. United States. https://doi.org/10.1210/jcem-47-5-967
Beall, G N, Chopra, I J, Solomon, D H, and Kruger, S R. 1978. "Serum protein inhibition of thyrotropin binding to human thyroid tissue. [/sup 125/I tracer technique]". United States. https://doi.org/10.1210/jcem-47-5-967.
@article{osti_6542704,
title = {Serum protein inhibition of thyrotropin binding to human thyroid tissue. [/sup 125/I tracer technique]},
author = {Beall, G N and Chopra, I J and Solomon, D H and Kruger, S R},
abstractNote = {We used a modificaton of the TSH radioreceptor assay to detect TSH-binding inhibition (TBI) activity in serum and serum fractions from normal subjects and patients with Graves' disease. TBI activity is present in normal IgG prepared by DEAE-Sephadex chromatography and in normal globulins prepared by precipitation at 1.6 M ammonium sulfate. Other normal serum proteins also had TBI activity when large concentrations were tested. Gel filtration chromatography and powder block electrophoresis were used to prepare fractions of normal and Graves' disease sera. In these fractions from normal serum, TBI activity was found in both ..gamma..-globulin and ..cap alpha..-globulin-albumin fractions electrophoretically and in both 7S and 4S peaks from gel filtration. TBI activity from Graves' disease patients' sera was similarly distributed, but relatively more TBI accompanied the electrophoretic ..gamma..-globulins. Sepharose Protein-A and anti-IgG were used as immunoabsorbents to isolate and purify IgG from normal and Graves' disease sera. TBI activity in IgG was proportional to the IgG concentration, indicating that the TBI which migrates as a ..gamma..-globulin electrophoretically is an IgG and thus may possibly be an antibody. Inhibitory activity found in normal serum globulins and in the non-IgG fractions of both normal and abnormal sera seriously interferes with attempts to use the TSH radioreceptor assay to study the hypothesized anti-TSH receptor antibody in the serum of patients with Graves' disease.},
doi = {10.1210/jcem-47-5-967},
url = {https://www.osti.gov/biblio/6542704}, journal = {J. Clin. Endocrinol. Metab.; (United States)},
number = ,
volume = 47:5,
place = {United States},
year = {Wed Nov 01 00:00:00 EST 1978},
month = {Wed Nov 01 00:00:00 EST 1978}
}