Nerve growth factor (NGF) induces neuronal differentiation in neuroblastoma cells transfected with the NGF receptor cDNA

Journal Article · Sat Sep 01 00:00:00 EDT 1990 · Molecular and Cellular Biology; (USA)

DOI:https://doi.org/10.1128/MCB.10.9.5015· OSTI ID:6532606

Matsushima, H; Bogenmann, E ^[1]

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1. Children's Hospital of Los Angeles, CA (USA)

Human nerve growth factor (NGF) receptor (NGFR) cDNA was transfected into a neuroblastoma cell line (HTLA 230) which does not express a functional NGF-NGFR signal transduction cascade. Short-term treatment of stably transfected cells (98-3) expressing membrane-bound NGF receptor molecules resulted in a cell cycle-dependent, transient expression of the c-fos gene upon treatment with NGF, suggesting the presence of functional high-affinity NGFR. Extensive outgrowth of neurites and cessation of DNA synthesis occurred in transfectants grown on an extracellular matrix after long-term treatment with NGF, suggesting terminal differentiation. Our data support the idea that introduction of a constitutively expressed NGFR cDNA into cells with neuronal background results in the assembly of a functional NGF-NGFR signal cascade in a permissive extracellular environment.

• OSTI ID: 6532606
• Journal Information: Molecular and Cellular Biology; (USA), Vol. 10:9; ISSN 0270-7306
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
GROWTH FACTORS
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RECEPTORS
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550201* - Biochemistry- Tracer Techniques