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Title: Casein gene expression in mouse mammary epithelial cell lines: Dependence upon extracellular matrix and cell type

Abstract

The COMMA-D mammary cell line exhibits mammary-specific functional differentiation under appropriate conditions in cell culture. The cytologically heterogeneous COMMA-D parental line and the clonal lines DB-1, TA-5, and FA-1 derived from the COMMA-D parent were examined for similar properties of functional differentiation. In monolayer cell culture, the cell lines DB-1, TA-5, FA-1, and MA-4 were examined for expression of mammary-specific and epithelial-specific proteins by an indirect immunofluorescence assay. The clonal cell lines were relatively homogeneous in their respective staining properties and seemed to represent three subpopulations found in the heterogeneous parental COMMA-D lines. None of the four clonal lines appeared to represent myoepithelial cells. The cell lines were examined for expression of {beta}-casein mRNA in the presence or absence of prolactin. The inducibility of {beta}-casein in the COMMA-D cell line was further enhanced by a reconstituted basement membrane preparation enriched in laminin, collagen IV, and proteoglycans. These results support the hypothesis that the functional response of inducible mammary cell populations is a result of interaction among hormones, multiple extracellular matrix components, and specific cell types.

Authors:
;  [1]; ;  [2]
  1. (Baylor College of Medicine, Houston, TX (USA))
  2. (Univ. of California, Berkeley (USA))
Publication Date:
OSTI Identifier:
6530901
DOE Contract Number:  
AC03-76SF00098
Resource Type:
Journal Article
Resource Relation:
Journal Name: Experimental Cell Research; (USA); Journal Volume: 172:1
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CASEIN; GENE REGULATION; EPITHELIUM; CYTOLOGICAL TECHNIQUES; CELL CULTURES; CELL DIFFERENTIATION; COLLAGEN; CULTURE MEDIA; GLYCOPROTEINS; HORMONES; LH; MAMMARY GLANDS; MESSENGER-RNA; MICE; ANIMAL TISSUES; ANIMALS; BODY; GLANDS; GONADOTROPINS; MAMMALS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; ORGANIC PHOSPHORUS COMPOUNDS; ORGANS; PEPTIDE HORMONES; PITUITARY HORMONES; PROTEINS; RNA; RODENTS; SCLEROPROTEINS; TISSUES; VERTEBRATES; 550301* - Cytology- Tracer Techniques

Citation Formats

Medina, D., Oborn, C.J., Li, M.L., and Bissell, M.J. Casein gene expression in mouse mammary epithelial cell lines: Dependence upon extracellular matrix and cell type. United States: N. p., 1987. Web. doi:10.1016/0014-4827(87)90105-4.
Medina, D., Oborn, C.J., Li, M.L., & Bissell, M.J. Casein gene expression in mouse mammary epithelial cell lines: Dependence upon extracellular matrix and cell type. United States. doi:10.1016/0014-4827(87)90105-4.
Medina, D., Oborn, C.J., Li, M.L., and Bissell, M.J. Tue . "Casein gene expression in mouse mammary epithelial cell lines: Dependence upon extracellular matrix and cell type". United States. doi:10.1016/0014-4827(87)90105-4.
@article{osti_6530901,
title = {Casein gene expression in mouse mammary epithelial cell lines: Dependence upon extracellular matrix and cell type},
author = {Medina, D. and Oborn, C.J. and Li, M.L. and Bissell, M.J.},
abstractNote = {The COMMA-D mammary cell line exhibits mammary-specific functional differentiation under appropriate conditions in cell culture. The cytologically heterogeneous COMMA-D parental line and the clonal lines DB-1, TA-5, and FA-1 derived from the COMMA-D parent were examined for similar properties of functional differentiation. In monolayer cell culture, the cell lines DB-1, TA-5, FA-1, and MA-4 were examined for expression of mammary-specific and epithelial-specific proteins by an indirect immunofluorescence assay. The clonal cell lines were relatively homogeneous in their respective staining properties and seemed to represent three subpopulations found in the heterogeneous parental COMMA-D lines. None of the four clonal lines appeared to represent myoepithelial cells. The cell lines were examined for expression of {beta}-casein mRNA in the presence or absence of prolactin. The inducibility of {beta}-casein in the COMMA-D cell line was further enhanced by a reconstituted basement membrane preparation enriched in laminin, collagen IV, and proteoglycans. These results support the hypothesis that the functional response of inducible mammary cell populations is a result of interaction among hormones, multiple extracellular matrix components, and specific cell types.},
doi = {10.1016/0014-4827(87)90105-4},
journal = {Experimental Cell Research; (USA)},
number = ,
volume = 172:1,
place = {United States},
year = {Tue Sep 01 00:00:00 EDT 1987},
month = {Tue Sep 01 00:00:00 EDT 1987}
}