Effects of various chemical agents on sister chromatid exchanges, chromosome aberrations, and DNA repair in normal and abnormal human lymphoid cell lines
Cells from a line (SN1029) derived from a case of chronic lymphocytic leukemia (CLL) were highly sensitive to the killing effects of 4-nitroquinoline 1-oxide (4-NQO), methyl methanesulfonate, ethyl methanesulfonate (EMS), mitomycin C (MMC), daunomycin (DM), bleomycin, and cytosine arabinoside, as compared to the lack of sensitivity of a normal lymphocytic cell line treated with these agents. Cells treated with N-methyl-N'-nitro-N-nitronitrosoguanidine (MNNG) were a possible exception to this pattern. Among the chemicals tested, SN1029 cells were extremely sensitive to MMC with regard to frequency of sister chromatid exchange(s) (SCE), even though MMC did not greatly increase the incidence of chromosome aberrations. Reverse results were obtained with the 6 other chemicals. Unscheduled DNA synthesis (USD) in normal and SN1029 cells was examined following uv radiation (200 ergs/mm/sup 2/) and treatments with EMS, MNNG, MMC, and 4-NQO for 90 min at 6.0 ..mu..g/ml (expt I) and for 48 hrs at 0.003, 0.03, 0.3, and 6.0 ..mu..g/ml (expt II). Almost no detectable levels of USD were seen with MMC in experiment I or with MMC, EMS, MNNG, and 4-NQO in experiment II, whereas USD did occur following 4-NQO in experiment II, whereas USD did occur following 4-NGO, EMS, MNNG, and uv radiation in experiment I. Comparison of SCE breakpoints and USD indicated that the SCE breakpoints within chromosome No. 1 in MMC- and 4-NQO-treated normal cells were nonrandom. The results indicate that SCE and excision repair are not closely linked and that the high SCE sensitivity and cell death ofthe CLL cell line are independent of the quantitative capacity for excision repair.
- Research Organization:
- Roswell Park Memorial Inst., Buffalo, NY
- OSTI ID:
- 6530225
- Journal Information:
- J. Natl. Cancer Inst.; (United States), Vol. 62:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
BIOLOGICAL REPAIR
BIOLOGICAL RADIATION EFFECTS
CARCINOGENS
CHROMOSOMAL ABERRATIONS
RADIOINDUCTION
CHROMOSOMES
DNA
LYMPHOCYTES
SISTER CHROMATID EXCHANGES
MUTAGENS
TUMOR CELLS
CELL CULTURES
EMS
IRRADIATION
LEUKEMIA
MAN
METHYL METHANESULFONATE
NITROSO COMPOUNDS
QUINOLINES
ULTRAVIOLET RADIATION
ANIMAL CELLS
ANIMALS
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RECOVERY
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DISEASES
ELECTROMAGNETIC RADIATION
ESTERS
HEMIC DISEASES
HETEROCYCLIC COMPOUNDS
LEUKOCYTES
MAMMALS
MUTATIONS
NEOPLASMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PRIMATES
PYRIDINES
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
SOMATIC CELLS
SULFONIC ACID ESTERS
VERTEBRATES
560121* - Radiation Effects on Cells- External Source- (-1987)
560301 - Chemicals Metabolism & Toxicology- Cells- (-1987)
550300 - Cytology