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Title: Whole-body autoradiographic distribution of exogenously administered renal renin in rats

Abstract

We studied, by whole-body autoradiography, the distribution of exogenously administered renal renin in rat. Rat renal renin was completely purified and labeled with /sup 125/I ((/sup 125/I)-renin) and was then injected into the tail veins of conscious rats at a dose of 30 microCi, 430 ng. After various intervals, rats were killed by an overdose of ether, the whole body rapidly frozen in acetone-dry ice, and autoradiography performed on sagittal whole-body sections. To remove breakdown products ((/sup 125/I)-tyrosine and free /sup 125/I) from (/sup 125/I)-renin, sections were treated with perchloric acid solution. The main accumulation of (/sup 125/I)-renin acid-insoluble radioactivity was observed in liver and renal cortex. The accumulation in these organs was already evident 2 min after the injection, reached a maximum level by 15 min, then gradually decreased. A small amount of (/sup 125/I)-renin was also evident in spleen, bone marrow, and adrenal gland. Thirty min after the injection, radioactivity began to appear in the thyroid gland, stomach, and small intestine, but disappeared with acid treatment, except in the thyroid. Radioactivity was negligible in other organs including brain, submaxillary gland, lung, heart, and testis. These autoradiographs clearly demonstrate that exogenously administered renal renin is distributed mainly in themore » liver and renal cortex.« less

Authors:
; ; ; ;
Publication Date:
Research Org.:
Osaka City Univ. Medical School, Japan
OSTI Identifier:
6529200
Resource Type:
Journal Article
Journal Name:
J. Histochem. Cytochem.; (United States)
Additional Journal Information:
Journal Volume: 5
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; BODY; AUTORADIOGRAPHY; IODINE 125; TISSUE DISTRIBUTION; RENIN; ADRENAL GLANDS; BONE MARROW; KIDNEYS; LIVER; PHARMACOLOGY; RATS; SMALL INTESTINE; SPLEEN; STOMACH; THYROID; ANIMAL TISSUES; ANIMALS; BETA DECAY RADIOISOTOPES; DAYS LIVING RADIOISOTOPES; DIGESTIVE SYSTEM; DISTRIBUTION; ELECTRON CAPTURE RADIOISOTOPES; ENDOCRINE GLANDS; ENZYMES; GASTROINTESTINAL TRACT; GLANDS; HEMATOPOIETIC SYSTEM; HYDROLASES; INTERMEDIATE MASS NUCLEI; INTESTINES; IODINE ISOTOPES; ISOTOPES; MAMMALS; NONSPECIFIC PEPTIDASES; NUCLEI; ODD-EVEN NUCLEI; ORGANS; PEPTIDE HYDROLASES; RADIOISOTOPES; RODENTS; TISSUES; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Kim, S, Iwao, H, Nakamura, N, Ikemoto, F, and Yamamoto, K. Whole-body autoradiographic distribution of exogenously administered renal renin in rats. United States: N. p., 1987. Web. doi:10.1177/35.5.3549890.
Kim, S, Iwao, H, Nakamura, N, Ikemoto, F, & Yamamoto, K. Whole-body autoradiographic distribution of exogenously administered renal renin in rats. United States. https://doi.org/10.1177/35.5.3549890
Kim, S, Iwao, H, Nakamura, N, Ikemoto, F, and Yamamoto, K. 1987. "Whole-body autoradiographic distribution of exogenously administered renal renin in rats". United States. https://doi.org/10.1177/35.5.3549890.
@article{osti_6529200,
title = {Whole-body autoradiographic distribution of exogenously administered renal renin in rats},
author = {Kim, S and Iwao, H and Nakamura, N and Ikemoto, F and Yamamoto, K},
abstractNote = {We studied, by whole-body autoradiography, the distribution of exogenously administered renal renin in rat. Rat renal renin was completely purified and labeled with /sup 125/I ((/sup 125/I)-renin) and was then injected into the tail veins of conscious rats at a dose of 30 microCi, 430 ng. After various intervals, rats were killed by an overdose of ether, the whole body rapidly frozen in acetone-dry ice, and autoradiography performed on sagittal whole-body sections. To remove breakdown products ((/sup 125/I)-tyrosine and free /sup 125/I) from (/sup 125/I)-renin, sections were treated with perchloric acid solution. The main accumulation of (/sup 125/I)-renin acid-insoluble radioactivity was observed in liver and renal cortex. The accumulation in these organs was already evident 2 min after the injection, reached a maximum level by 15 min, then gradually decreased. A small amount of (/sup 125/I)-renin was also evident in spleen, bone marrow, and adrenal gland. Thirty min after the injection, radioactivity began to appear in the thyroid gland, stomach, and small intestine, but disappeared with acid treatment, except in the thyroid. Radioactivity was negligible in other organs including brain, submaxillary gland, lung, heart, and testis. These autoradiographs clearly demonstrate that exogenously administered renal renin is distributed mainly in the liver and renal cortex.},
doi = {10.1177/35.5.3549890},
url = {https://www.osti.gov/biblio/6529200}, journal = {J. Histochem. Cytochem.; (United States)},
number = ,
volume = 5,
place = {United States},
year = {Fri May 01 00:00:00 EDT 1987},
month = {Fri May 01 00:00:00 EDT 1987}
}