Effects of murine natural killer cells on Cryptococcus neoformans

Thesis/Dissertation · Tue Jan 01 00:00:00 EST 1985

OSTI ID:6526518

Nabavi Nouri, N

Previous data generated by Murphy and McDaniel indicate that normal murine nylon wool nonadherent splenic cells, with the characteristics of natural killer (NK) cells, effectively inhibit the in vitro growth of Cryptococcus neoformans, a yeast-like pathogen. Nylon wood nonadherent cells from spleens of 7-8 week old mice were further fractionated on discontinuous Percoll gradients. The enrichment of NK cells in Percoll fractions 1 and 2 was confirmed by morphological examination, immunofluorescent staining, and by assessing the cytolytic activity of each Percoll cell fraction against YAC-1 targets in the 4 h /sup 51/Cr release assay. Cells isolated from each Percoll fraction were tested for growth inhibitory activity against C neoformans, using an in vitro 18 h growth inhibition assay. The results showed that NK cell enrichment was concomitant with the enrichment of anti-cryptococcal activity the Percoll fractions 1 and 2. An immunolabeling method combined with scanning electron microscopy was used to demonstrate that the effector cells attached to C. neoformans were asialo GM/sub 1/ positive and, therefore, had NK cell characteristics. NK cells have Fc receptors on their surfaces , and are capable of antibody-dependent cell-mediated cytotoxicity (ADCC) against IgG-coated target cells. The author examined the effects of the IgG fraction of rabbit anti-cryptococcal antibody on the NK cell-mediated growth inhibition of C. neoformans. The data indicated that the effector cells involved in antibody-dependent growth inhibition of cryptococci are either NK cells or copurify and coexist in the same population with NK cells.

• Research Organization: Oklahoma Univ., Norman (USA)
• OSTI ID: 6526518
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GROWTH
INHIBITION
SPLEEN CELLS
TOXICITY
CELL KILLING
CHROMIUM 51
MICE
PATHOGENS
RECEPTORS
SCANNING ELECTRON MICROSCOPY
YEASTS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
CHROMIUM ISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ELECTRON MICROSCOPY
EVEN-ODD NUCLEI
FUNGI
INTERMEDIATE MASS NUCLEI
ISOTOPES
MAMMALS
MEMBRANE PROTEINS
MICROORGANISMS
MICROSCOPY
NUCLEI
ORGANIC COMPOUNDS
PLANTS
PROTEINS
RADIOISOTOPES
RODENTS
SOMATIC CELLS
VERTEBRATES
550301* - Cytology- Tracer Techniques