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Title: Factors affecting the photoproduction of ammonia from dinitrogen and water by the cyanobacterium Anabaena sp. strain ATCC 33047

Abstract

Synthesis of ammonia from dinitrogen and water by suspensions of Anabaena sp. strain ATCC 33047 treated with the glutamine synthetase inhibitor L-methionine-D,L-sulfoximine is strictly dependent on light. Under otherwise optimal conditions, the yield of ammonia production is influenced by irradiance, as well as by the density, depth, and turbulence of the cell suspension. The interaction among these factors seems to determine the actual amount of light available to each single cell or filament in the suspension for the photoproduction process. Under convenient illumination, the limiting factor in the synthesis of ammonia seems to be the cellular nitrogenase activity level, but under limiting light conditions the limiting factor could, however, be the assimilatory power required for nitrogen fixation. Photosynthetic ammonia production from atmospheric nitrogen and water can operate with an efficiency of ca. 10% of its theoretical maximum, representing a remarkable process for the conversion of light energy into chemical energy.

Authors:
; ;
Publication Date:
Research Org.:
CSIC, Sevilla, Spain
OSTI Identifier:
6526332
Resource Type:
Journal Article
Journal Name:
Biotechnol. Bioeng.; (United States)
Additional Journal Information:
Journal Volume: 29:5
Country of Publication:
United States
Language:
English
Subject:
14 SOLAR ENERGY; AMMONIA; SYNTHESIS; CYANOBACTERIA; PHOTOSYNTHESIS; NITROGENASE; ENZYME ACTIVITY; ENZYME INHIBITORS; NITROGEN; VISIBLE RADIATION; WATER; CHEMICAL REACTIONS; ELECTROMAGNETIC RADIATION; ELEMENTS; ENZYMES; HYDRIDES; HYDROGEN COMPOUNDS; MICROORGANISMS; NITRO-GROUP DEHYDROGENASES; NITROGEN COMPOUNDS; NITROGEN HYDRIDES; NONMETALS; OXIDOREDUCTASES; OXYGEN COMPOUNDS; PHOTOCHEMICAL REACTIONS; RADIATIONS; 140505* - Solar Energy Conversion- Photochemical, Photobiological, & Thermochemical Conversion- (1980-)

Citation Formats

Ramos, J L, Guerrero, M G, and Losada, M. Factors affecting the photoproduction of ammonia from dinitrogen and water by the cyanobacterium Anabaena sp. strain ATCC 33047. United States: N. p., 1987. Web. doi:10.1002/bit.260290505.
Ramos, J L, Guerrero, M G, & Losada, M. Factors affecting the photoproduction of ammonia from dinitrogen and water by the cyanobacterium Anabaena sp. strain ATCC 33047. United States. https://doi.org/10.1002/bit.260290505
Ramos, J L, Guerrero, M G, and Losada, M. 1987. "Factors affecting the photoproduction of ammonia from dinitrogen and water by the cyanobacterium Anabaena sp. strain ATCC 33047". United States. https://doi.org/10.1002/bit.260290505.
@article{osti_6526332,
title = {Factors affecting the photoproduction of ammonia from dinitrogen and water by the cyanobacterium Anabaena sp. strain ATCC 33047},
author = {Ramos, J L and Guerrero, M G and Losada, M},
abstractNote = {Synthesis of ammonia from dinitrogen and water by suspensions of Anabaena sp. strain ATCC 33047 treated with the glutamine synthetase inhibitor L-methionine-D,L-sulfoximine is strictly dependent on light. Under otherwise optimal conditions, the yield of ammonia production is influenced by irradiance, as well as by the density, depth, and turbulence of the cell suspension. The interaction among these factors seems to determine the actual amount of light available to each single cell or filament in the suspension for the photoproduction process. Under convenient illumination, the limiting factor in the synthesis of ammonia seems to be the cellular nitrogenase activity level, but under limiting light conditions the limiting factor could, however, be the assimilatory power required for nitrogen fixation. Photosynthetic ammonia production from atmospheric nitrogen and water can operate with an efficiency of ca. 10% of its theoretical maximum, representing a remarkable process for the conversion of light energy into chemical energy.},
doi = {10.1002/bit.260290505},
url = {https://www.osti.gov/biblio/6526332}, journal = {Biotechnol. Bioeng.; (United States)},
number = ,
volume = 29:5,
place = {United States},
year = {1987},
month = {4}
}