Use of antibodies specific to defined regions of scorpion. cap alpha. -toxin to study its interaction with its receptor site on the sodium channel
Five antibody populations selected by immunoaffinity chromatography for the specificity toward various regions of toxin II of the scorpion Androctonus australis Hector were used to probe the interaction of this protein with its receptor site on the sodium channel. These studies indicate that two antigenic sites, one located around the disulfide bridge 12-63 and one encompassing residues 50-59, are involved in the molecular mechanisms of toxicity neutralization. Fab fragments specific to the region around disulfide bridge 12-63 inhibit binding of the /sup 125/I-labeled toxin to its receptor site. Also, these two antigenic regions are inaccessible to the antibodies when the toxin is bound to its receptor site. In contrast, the two other antigenic sites encompassing the only ..cap alpha..-helix region (residues 23-32) and a ..beta..-turn structure (residues 32-35) are accessible to the respective antibodies when the toxin is bound to its receptor. Together, these data support the recent proposal that a region made of residues that are conserved in the scorpion toxin family is involved in the binding of the toxin to the receptor.
- Research Organization:
- INSERM U172, Marseille, France
- OSTI ID:
- 6523631
- Journal Information:
- Biochemistry; (United States), Vol. 25:21
- Country of Publication:
- United States
- Language:
- English
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TOXINS
MOLECULAR STRUCTURE
RADIORECEPTOR ASSAY
ANTIBODIES
ANTIGEN-ANTIBODY REACTIONS
CATIONS
IMMUNOGLOBULINS
IODINE 125
ION EXCHANGE CHROMATOGRAPHY
SCORPIONS
SODIUM COMPOUNDS
SODIUM IODIDES
ALKALI METAL COMPOUNDS
ANIMALS
ANTIGENS
ARACHNIDS
ARTHROPODS
BETA DECAY RADIOISOTOPES
CHARGED PARTICLES
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
GLOBULINS
HALIDES
HALOGEN COMPOUNDS
INORGANIC PHOSPHORS
INTERMEDIATE MASS NUCLEI
INVERTEBRATES
IODIDES
IODINE COMPOUNDS
IODINE ISOTOPES
IONS
ISOTOPE APPLICATIONS
ISOTOPES
MATERIALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PHOSPHORS
PROTEINS
RADIOISOTOPES
SEPARATION PROCESSES
TOXIC MATERIALS
TRACER TECHNIQUES
550601* - Medicine- Unsealed Radionuclides in Diagnostics