Effect of cysteamine on cytosolic somatostatin binding sites in rabbit duodenal mucosa

Gonzalez-Guijarro, L; Lopez-Ruiz, M P; Bodegas, G; Prieto, J C; Arilla, E

doi:10.1016/0014-4800(87)90061-X

Title: Effect of cysteamine on cytosolic somatostatin binding sites in rabbit duodenal mucosa

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Abstract

Administration of cysteamine in rabbits elicited a rapid depletion of both duodenal mucosa and plasma somatostatin. A significant reduction was observed within 5 min, returning toward control values by 150 min. The depletion of somatostatin was associated with an increase in the binding capacity and a decrease in the affinity of both high- and low-affinity binding sites present in cytosol of duodenal mucosa. Incubation of cytosolic fraction from control rabbits with 1 mM cysteamine did not modify somatostatin binding. Furthermore, addition of cysteamine at the time of binding assay did not affect the integrity of /sup 125/I-Tyr11-somatostatin. It is concluded that in vivo administration of cysteamine to rabbits depletes both duodenal mucosa and plasma somatostatin and leads to up-regulation of duodenal somatostatin binding sites.

Authors:: Gonzalez-Guijarro, L; Lopez-Ruiz, M P; Bodegas, G; Prieto, J C; Arilla, E

Publication Date:: Wed Apr 01 00:00:00 EST 1987

Research Org.:: Universidad de Alcala de Henares, Madrid, Spain

OSTI Identifier:: 6518429

Resource Type:: Journal Article

Journal Name:: Exp. Mol. Pathol.; (United States)

Additional Journal Information:: Journal Volume: 2

Country of Publication:: United States

Language:: English

Subject:: 63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; MEA; TOXICITY; SOMATOSTATIN; BIOCHEMICAL REACTION KINETICS; IODINE 125; IODINE COMPOUNDS; MUCOUS MEMBRANES; RABBITS; SMALL INTESTINE; TRACER TECHNIQUES; AMINES; ANIMALS; BETA DECAY RADIOISOTOPES; BODY; DAYS LIVING RADIOISOTOPES; DIGESTIVE SYSTEM; DRUGS; ELECTRON CAPTURE RADIOISOTOPES; GASTROINTESTINAL TRACT; HALOGEN COMPOUNDS; INTERMEDIATE MASS NUCLEI; INTESTINES; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; KINETICS; MAMMALS; MEMBRANES; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANIC SULFUR COMPOUNDS; ORGANS; RADIOISOTOPES; RADIOPROTECTIVE SUBSTANCES; REACTION KINETICS; THIOLS; VERTEBRATES; 560151* - Radiation Effects on Animals- Man

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                    Gonzalez-Guijarro, L, Lopez-Ruiz, M P, Bodegas, G, Prieto, J C, and Arilla, E. Effect of cysteamine on cytosolic somatostatin binding sites in rabbit duodenal mucosa.  United States: N. p., 1987. 
        Web.  doi:10.1016/0014-4800(87)90061-X.

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                    Gonzalez-Guijarro, L, Lopez-Ruiz, M P, Bodegas, G, Prieto, J C, & Arilla, E. Effect of cysteamine on cytosolic somatostatin binding sites in rabbit duodenal mucosa.  United States.  https://doi.org/10.1016/0014-4800(87)90061-X

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                    Gonzalez-Guijarro, L, Lopez-Ruiz, M P, Bodegas, G, Prieto, J C, and Arilla, E. 1987.  
        "Effect of cysteamine on cytosolic somatostatin binding sites in rabbit duodenal mucosa".  United States.  https://doi.org/10.1016/0014-4800(87)90061-X.

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@article{osti_6518429,

  title        = {Effect of cysteamine on cytosolic somatostatin binding sites in rabbit duodenal mucosa},

  author       = {Gonzalez-Guijarro, L and Lopez-Ruiz, M P and Bodegas, G and Prieto, J C and Arilla, E},

  abstractNote = {Administration of cysteamine in rabbits elicited a rapid depletion of both duodenal mucosa and plasma somatostatin. A significant reduction was observed within 5 min, returning toward control values by 150 min. The depletion of somatostatin was associated with an increase in the binding capacity and a decrease in the affinity of both high- and low-affinity binding sites present in cytosol of duodenal mucosa. Incubation of cytosolic fraction from control rabbits with 1 mM cysteamine did not modify somatostatin binding. Furthermore, addition of cysteamine at the time of binding assay did not affect the integrity of /sup 125/I-Tyr11-somatostatin. It is concluded that in vivo administration of cysteamine to rabbits depletes both duodenal mucosa and plasma somatostatin and leads to up-regulation of duodenal somatostatin binding sites.},

  doi          = {10.1016/0014-4800(87)90061-X},

  url          = {https://www.osti.gov/biblio/6518429},
  journal      = {Exp. Mol. Pathol.; (United States)},
number       = ,

  volume       = 2,

  place        = {United States},

  year         = {Wed Apr 01 00:00:00 EST 1987},

  month        = {Wed Apr 01 00:00:00 EST 1987}

}

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